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Imp/IGF2BP levels modulate individual neural stem cell growth and division through myc mRNA stability

ABSTRACT: Imp/IGF2BP levels modulate individual neural stem cell growth and division through myc mRNA stability

PROVIDER: PRJNA591594 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10529519.fastq.gz	Fastqsanger.gz
	SRR10529520.fastq.gz	Fastqsanger.gz
	SRR10529521.fastq.gz	Fastqsanger.gz
	SRR10529522.fastq.gz	Fastqsanger.gz
	SRR10529523.fastq.gz	Fastqsanger.gz

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Imp/IGF2BP levels modulate individual neural stem cell growth and division through myc mRNA stability

Project description:The numerous neurons and glia that form the brain originate from tightly controlled growth and division of neural stem cells, regulated systemically by known extrinsic signals. However, the intrinsic mechanisms that control the characteristic proliferation rates of individual neural stem cells are unknown. Here, we show that the size and division rates of Drosophila neural stem cells (neuroblasts) are controlled by the highly conserved RNA binding protein Imp (IGF2BP), via one of its top binding targets in the brain, myc mRNA. We show that Imp stabilises myc mRNA leading to increased Myc protein levels, larger neuroblasts, and faster division rates. Declining Imp levels throughout development limit myc mRNA stability to restrain neuroblast growth and division, while heterogeneous Imp expression correlates with myc mRNA stability between individual neuroblasts in the brain. We propose that Imp-dependent regulation of myc mRNA stability fine-tunes individual neural stem cell proliferation rates.

2020-02-05 | GSE140704 | GEO

Imp/IGF2BP levels modulate individual neural stem cell growth and division through myc mRNA stability.

Project description:The numerous neurons and glia that form the brain originate from tightly controlled growth and division of neural stem cells, regulated systemically by important known stem cell-extrinsic signals. However, the cell-intrinsic mechanisms that control the distinctive proliferation rates of individual neural stem cells are unknown. Here, we show that the size and division rates of Drosophila neural stem cells (neuroblasts) are controlled by the highly conserved RNA binding protein Imp (IGF2BP), via one of its top binding targets in the brain, myc mRNA. We show that Imp stabilises myc mRNA leading to increased Myc protein levels, larger neuroblasts, and faster division rates. Declining Imp levels throughout development limit myc mRNA stability to restrain neuroblast growth and division, and heterogeneous Imp expression correlates with myc mRNA stability between individual neuroblasts in the brain. We propose that Imp-dependent regulation of myc mRNA stability fine-tunes individual neural stem cell proliferation rates.

| S-EPMC7025822 | biostudies-literature

The RNA interactomes of Imp and Syp reveal temporal RNA regulatory network in Drosophila brain development

Project description:The temporal patterning of neural stem cells is a powerful mechanism to generate neural diversity. In Drosophila, the progression of post-embryonic neurogenesis is driven by RNA-binding proteins Imp (IGF2BP) and Syp (SYNCRIP), however how they achieve their function is not well understood, since little is known about their RNA targets in the brain. Here, we present comprehensive RNA interactomes of Imp and Syp at different time points during larval brain development. Imp and Syp target highly overlapping sets of mRNAs whose encoded proteins impact fate specification, stem cell maintenance and tumourigenesis. Within these transcripts, Imp and Syp footprints exhibit limited overlap, instead we show their binding sites have co-evolved, suggesting a combinatorial or cooperative mode of regulation rather than competitive binding. Further, we identify RNAs dynamically interacting with Imp/Syp across development, consisting of existing and potentially new candidates of the neurogenesis program, and loss of Imp or Syp disrupts expression of key transcripts denotative of early or late-born neurons. Together, our findings highlight a post-transcriptional regulatory network that influences temporal order, and our study is a valuable resource for future studies finely-dissecting molecular mechanisms of neuronal fate specifications.

2025-01-16 | GSE262499 | GEO

Visualization of individual cell division history in complex tissues

Project description:The division potential of individual stem cells and the molecular consequences of successive rounds of proliferation remain largely unknown. Here, we developed an inducible cell division counter (iCOUNT) that reports cell division events in human and mouse tissues in vitro and in vivo. Analysing cell division histories of neural stem/progenitor cells (NSPCs) in the developing and adult brain, we show that iCOUNT can provide novel insights into stem cell behaviour. Further, we used single cell RNA-sequencing (scRNA-seq) of iCOUNT-labelled NSPCs and their progenies from the developing mouse cortex and forebrain-regionalized human organoids to identify molecular pathways that are commonly regulated between mouse and human cells, depending on individual cell division histories. Thus, we developed a novel tool to characterize the molecular consequences of repeated cell divisions of stem cells that allows an analysis of the cellular principles underlying tissue formation, homeostasis, and repair.

2021-07-19 | GSE167375 | GEO

E. coli imp fabI(G93V) Mutant Cells: Control (E. coli imp fabI(G93V) ) vs. NTG-treated E. coli imp fabI(G93V)

Project description:Transcriptional profiling of NTG-treated E. coli imp fabI(G93V) cells compared to control cells (E. coli imp fabI(G93V) ) in the absence or presence of sub-lethal concentration of triclosan.

2009-07-31 | GSE17441 | GEO

Visualization of individual cell division history in complex tissues

Project description:Visualization of individual cell division history in complex tissues

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MeWo/IMP-3 v.s. MeWo/vec

Project description:IMP-3 Promotes Migration and Invasion of Melanoma Cells by Modulating the Expression of HMGA2 and Predicts Poor Prognosis in Melanoma Two-condition experiment,MeWo/IMP-3 v.s. MeWo/vec. Biological replicates: 2 control replicates, 2 transfected replicates.

2014-10-23 | E-GEOD-62574 | biostudies-arrayexpress

The let-7-Imp axis regulates aging of the Drosophila testis stem cell niche.

Project description:Adult stem cells support tissue homeostasis and repair throughout the life of an individual. However, numerous intrinsic and extrinsic changes occur with age that result in altered stem cell behavior and reduced tissue maintenance and regeneration. In the Drosophila testis, stem cells surround and contact the apical hub, a cluster of somatic cells that express the self-renewal factor Unpaired (Upd), which activates the JAK-STAT pathway in adjacent stem cells. However, aging results in a dramatic decrease in upd expression, with a concomitant loss of germline stem cells (GSCs). Here we present genetic and biochemical data to demonstrate that IGF-II mRNA binding protein (Imp) counteracts endogenous small interfering RNAs to stabilize upd RNA and contribute to maintenance of the niche. However, Imp expression decreases in hub cells of older males, similar to upd, which is due to targeting of Imp by the heterochronic microRNA let-7. Therefore, in the absence of Imp, upd mRNA becomes unprotected and susceptible to degradation. Understanding the mechanistic basis for aging-related changes in stem cell behavior will lead to the development of strategies to treat age-onset diseases and facilitate stem cell based therapies in older individuals.

2012-05-31 | GSE37041 | GEO

MeWo/IMP-3 v.s. MeWo/vec

Project description:IMP-3 Promotes Migration and Invasion of Melanoma Cells by Modulating the Expression of HMGA2 and Predicts Poor Prognosis in Melanoma

2014-10-23 | GSE62574 | GEO

IMP-producing Pseudomonas aeruginosa

Project description:IMP-producing Pseudomonas aeruginosa

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