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Effect of metformin on mRNA expression in lung cancer cells

ABSTRACT: Effect of metformin on mRNA expression in lung cancer cells

PROVIDER: PRJNA591888 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10534450_1.fastq.gz	Fastqsanger.gz
	SRR10534450_2.fastq.gz	Fastqsanger.gz
	SRR10534451_1.fastq.gz	Fastqsanger.gz
	SRR10534451_2.fastq.gz	Fastqsanger.gz

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MicroRNA profiling using microarrays of two human metformin-treated non-small cell lung cancer cell-lines

Project description:To examine the effect of metformin on lung cancer biology, human lung H226 and H1299 squamous cell carcinoma cell-lines were grown in RPMI-1640 medium with 10% v/v fetal bovine serum and with or without 15 uM metformin hydrochloride for 7-8 days. Medium (with any metformin) was replaced every two days. Paired cultures with and without metformin were grown and maintained in parallel. Three separate paired cultures, all seeded with same stock of frozen cells, were grown.

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Project description:The genes whose transcription was significantly altered by metformin were investigated in H1299 cells by RNA-seq. Significantly up- or down-regulated genes were identified by cutoff value of |FC| >= 1.5. Metformin significantly downregulated mRNA levels of 499 genes while upregulating 615. KEGG (Kyoto Encyclopedia of Genes and Genomes) gene enrichment analysis revealed that the highly ranked clusters were cell cycle, apoptosis, cellular senescence and p53 signaling pathways. Multiple cell cycle checkpoint genes such as CCNA, CCNB, CCNE, CDK1, E2F2, E2F6, and E2F8 were downregulated, while certain cell cycle inhibitors such as CDKN2B (p15), CDKN1A (p21), DDIT4, and GADD45 were upregulated.

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