Project description:microRNAs are small non-coding RNAs, which promote either mRNAs degradation or block their translation through binding to partially complementary sequences of their target mRNAs. We have previously isolated a subpopulation with accelerated baseline motility (MG cells) or an immotile one (non-MG cells) from a colon cancer cell line (HCT116). In this study, using these subpopulations, we identified miRNAs, which were involved in acquisition of a cell migratory phenotype.
Project description:Gene expression profiles were generated using RNA-sequencing from migratory and non-migratory B-cells after exposure to conditioned medium of breast cancer cells, either containing or depleted from extracellular vesicles. The purpose of the experiment was to investigate how breast cancer cell derived extracellular vesicles induce specific molecular pathways involved in B-cell migration and B-cell infiltration.
Project description:miRNA expression analysis was performed on 14 cases of high grade gliomas and 4 controls obtained from age-matched epileptic patients. After this differentially miRNA analysis has been done among T53,H3F3A,normal and pediatric high grade glioma patient and try to identify the highly altered miRNA expression as well as sno expression pattern.