Project description:For improving our understanding of the metabolic capacity of the bacterial genus Tepidiforma, quantitative exometabolomics was performed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) on pure cultures of two novel Tepidiforma species grown on R2A broth. Experimental samples were compared to sterile controls of the growth medium, either stored at 4 degrees C in the dark or incubated along with the culture tubes to account for thermal degradation of medium components. This dataset contains LC-MS/MS spectra of the sample filtrates.
Project description:Based on the genome sequencing and gene annotation, we analyzed the alginate lyases and other genes related to alginate metabolism of strain Vibrio sp. C42, with the highest alginate-degrading activity. Combined with proteome measurement, we proposed its alginate metabolic pathways.
Project description:Leishmania donovani WHO reference strain MHOM/IN/80/DD8 and Leptomonas seymouri isolates Ld 2001 and Ld39 were used for proteome analysis which were originally isolated from clinical cases of kala azar patients with different inherent antimonial sensitivities. Ld 2001 was Sb-S and Ld 39 was Sb-R. The genome sequencing of these isolates had confirmed co-infection with Leptomonas.