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Florigen family chromatin recruitment, competition and targets [RNA-Seq]

ABSTRACT: Florigen family chromatin recruitment, competition and targets [RNA-Seq]

PROVIDER: PRJNA595115 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10676638.fastq.gz	Fastqsanger.gz
	SRR10676639.fastq.gz	Fastqsanger.gz
	SRR10676640.fastq.gz	Fastqsanger.gz
	SRR10676642.fastq.gz	Fastqsanger.gz
	SRR10676643.fastq.gz	Fastqsanger.gz

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Florigen family chromatin recruitment, competition and targets

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Florigen family chromatin recruitment, competition and targets [ChIP-Seq]

Project description:This study identified direct targets of TFL1 and FD and showed that TFL1 is recruited to the chromatin by FD. We performed ChIP-seq using anti-GFP antibodies in gTFL1-GFP tfl1-1, gTFL1-GFP fd-1 and controls (nontransgenic plants). We used anti-GUS antibodies for gFD-GUS fd-1. ChIP was conducted on dissected, non-bolted, inflorescences from 42-day-old short-day-grown Arabidopsis thaliana.

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Florigen family chromatin recruitment, competition and targets

Project description:This SuperSeries is composed of the SubSeries listed below.

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Florigen family chromatin recruitment, competition and targets [RNA-Seq]

Project description:This study identified genes that re-upregulated in far-red enriched photoperiod in the wild type or tfl1-1, but not in ft-10 (FT-dependent photoinduction). We performed RNA seq on tfl1-1, wild type and ft-10 grown for 42 days in short-day conditions and treated for 24 hours with far-red enriched photoinduction (24 hr FRP) or grown for 43 days in short-day conditions (no FRP). RNA was isolated from trimmed and dissected non-bolted inflorescences.

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Florigen activation complex forms via multifaceted assembly in Arabidopsis

Project description:'Florigen', encoded by FT genes, is synthesized in leaves and transported to the shoot apical meristem (SAM) to induce flower development. At the SAM, 14-3-3 proteins are proposed to act as receptors for FT and to mediate the indirect interaction between FT and the bZIP transcription factor FD to form the 'florigen activation complex' (FAC) that activates transcription of flowering genes. We demonstrate a different mechanism of FAC assembly, diverse functions for the 14-3-3s within the complex and an unexpected spatiotemporal distribution of the FAC. We show that FT is not recruited by 14-3-3 alone, but that it interacts with the DNA−FD−14-3-3 complex through two interfaces, one of which binds DNA via the unstructured C-terminus of FT. Also, we find that interaction of 14-3-3s with the phosphorylated FD C-terminus reduces liquid-phase condensation of the intrinsically disordered FD protein allowing it to bind DNA, and that the 14-3-3s strengthen DNA binding of FD by promoting dimerization, which ultimately results in the recruitment of FT. Unexpectedly, we also discovered that after FT movement to the shoot apex, FT and FD are co-transcribed in young floral primordia forming a boundary with the suppressed bract and allowing formation of the FAC during the first stages of floral development. Our studies propose a new mechanism by which the florigen FT transcriptional complex is formed, and indicate distinct functions for the complex during SAM and floral primordium development.

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Florigen Accelerates Secondary Cell Wall Biogenesis to Harmonize Vascular Maturation with flowering and reproductive phase Development

Project description:The protein hormone florigen is a universal systemic inducer of flowering and a generic growth terminator across meristems. To understand the developmental rational for its pleiotropic functions and to uncover the deep cellular systems mobilized by florigen beyond flowering we explored the radial expansion of tomato stems. RNAseq, genetic analysis and histological validations show that endogenous, mobile, or induced florigen accelerate secondary cell wall biogenesis (SCWB), and hence vascular maturation, in coordination with but independently of flowering. Conversely, a systemic FT-like florigen antagonist from Ginkgo biloba, arrests SCWB. Downstream of florigen, RNAseq identified MADS/FUL and MIF genes, that similarly impact SCWB independent from flowering. Florigen we show is remarkably stable and distributed to all organs regardless of pre-existing endogenous levels. By accelerating SCWB, florigen triggers a global redistribution of resources, signals and mechanical loads, thereby harmonizing vascular maturation with the reproductive development it had originally set into motion.

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Dynamic Recruitment of miRNAs to their mRNA Targets in Regenerating Liver

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OsFD4 promotes the rice floral transition via florigen activation complex formation in the shoot apical meristem

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Dynamic Recruitment of miRNAs to their mRNA Targets in Regenerating Liver

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