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ABSTRACT: RNA-seq of porcine trophectoderm cells

PROVIDER: PRJNA598068 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10802146.fastq.gz	Fastqsanger.gz
	SRR10802147.fastq.gz	Fastqsanger.gz
	SRR10802148.fastq.gz	Fastqsanger.gz
	SRR10802149.fastq.gz	Fastqsanger.gz
	SRR10802150.fastq.gz	Fastqsanger.gz

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Project description:RNA-Seq of porcine trophectoderm cells

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Melatonin enhances function of porcine immature Sertoli cells via modulating gene expression and metabolism

Project description:<p>Melatonin (MT), a neurohormone synthesized and secreted primarily by the pineal gland, is of vital function to animal reproduction. However, the effect of MT treatment on porcine immature Sertoli cells (iSCs) remains unclear. Here, MT treatment (10 nM, 36 h) could elevate mitochondrial function and reduce oxidative stress, therefore inhibit apoptosis of porcine iSCs. Transcriptome profiling identified 39 differentially expressed genes (DEGs) (33 known and 6 novel) (MT vs Control), mainly involved in GO terms of steroid metabolic process, glutamine metabolic process, oxidoreductase activity and G protein coupled receptor binding, and KEGG pathways of steroid biogenesis, pyruvate metabolism, NF-kappa B and AMPK signal pathways. RT-qPCR validated 6 DEGs (Phgdh, Scd, Hmgcs1, Cytb, Pck2 and Sqle) induced by MT to be with the similar change trend to RNA-seq results. The protein level of HMGCS1 and the estradiol level were confirmed to be significantly decreased by MT (10 nM, 36 h) treatment of porcine iSCs. Inhibition of HMGCS1 in porcine iSCs could significantly reduce the level of estradiol. The levels of cholesterol content within cells and lactate in culture medium were unchanged by MT (10 nM, 36 h). 14 metabolites were significantly altered by MT (10 nM, 36 h) treatment of porcine iSCs. Which were involved in multiple vital metabolic pathways. Collectively, MT treatment of porcine iSCs could promote functions of porcine iSCs via modulating gene expression and metabolism.</p>

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RNA-seq of XTE cells and vesicles, XEN and XEN-like cells, and ES cells

Project description:The conventional trophoblast stem cells represent post-implantation extraembryonic ectoderm-like state and not that of pre-implantation trophectoderm, irrespective of their origin. To capture the trophectoderm-like state we established culture conditions that facilitate the co-existence of characteristics of both the extraembryonic lineages, namely, primitive endoderm and trophectoderm. We term these cells XTE cells. We carried out RNA-seq on XTE cells from different origins, as well as XEN cells, XEN-like cells, and ES cells, to assess their transcriptional profiles and identify putative marker genes and master regulators.

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Cycloleucine disturbs functions of porcine Sertoli cells via modulating gene expression and metabolism

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