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Epithelial planar bipolarity emerges from Notch- mediated asymmetric inhibition of Emx2

ABSTRACT: Epithelial planar bipolarity emerges from Notch- mediated asymmetric inhibition of Emx2

PROVIDER: PRJNA601204 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10887500_1.fastq.gz	Fastqsanger.gz
	SRR10887500_2.fastq.gz	Fastqsanger.gz
	SRR10887600_1.fastq.gz	Fastqsanger.gz
	SRR10887600_2.fastq.gz	Fastqsanger.gz
	SRR10887501_1.fastq.gz	Fastqsanger.gz

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Epithelial planar bipolarity emerges from Notch-mediated asymmetric inhibition of Emx2

Project description:Epithelial planar bipolarity emerges from Notch-mediated asymmetric inhibition of Emx2

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Epithelial planar bipolarity emerges from Notch- mediated asymmetric inhibition of Emx2

Project description:This dataset consists of single-cell RNA-seq (mcSCRBseq) data of neuromast hair cells from wild type and Emx2 mutant zebrafish larvae. Posterior lateral-line neuromast hair cells were isolated by fluorescence activated cell sorting (FACS) from the dissociated trunks of wild type and Emx2-mutant Tg[myo6b:actb1-EGFP] transgenic zebrafish larvae expressing the green fluorescent protein EGFP in hair cells and unipotent hair-cell progenitors (UHCPs).

2020-03-05 | GSE143663 | GEO

Epithelial planar bipolarity emerges from Notch-mediated asymmetric inhibition of Emx2

Project description:This dataset consists of single-cell RNA-seq (10X) data of neuromast cells from zebrafish larvae. Posterior lateral-line neuromast cells were isolated by fluorescence activated cell sorting (FACS) from the dissociated trunks of Tg[myo6b:actb1-EGFP] transgenic zebrafish larvae expressing the green fluorescent protein (EGFP) in hair cells and unipotent hair-cell progenitors (UHCPs), and from Tg[Et(krt4:EGFP)sqgw57A] larvae expressing EGFP in supporting cells.

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APT1 regulates the asymmetric partitioning of Notch and Wnt signaling during cell division

Project description:Asymmetric cell division results in two distinctly fated daughter cells to generate cellular diversity. A major molecular hallmark of an asymmetric division is the unequal partitioning of cell-fate determinant proteins. We have previously established that growth factor signaling promotes protein depalmitoylation to foster polarized protein localization, which in turns drives migration and metastasis. Here, we report protein palmitoylation as a key mechanism for the asymmetric partitioning of the cell-fate determinants Numb (Notch antagonist) and β-catenin (canonical Wnt regulator) through the activity of a depalmitoylating enzyme, APT1. Using point mutants, we show specific palmitoylated residues on proteins, such as Numb, are required for asymmetric localization. Furthermore, by live-cell imaging, we show that reciprocal interactions between APT1 and CDC42 regulate the asymmetric localization of Numb and β-catenin to the plasma membrane. This in turn restricts Notch and Wnt transcriptional activity to one daughter cell. Moreover, we show altering APT1 expression changes the transcriptional signatures to those resembling that of Notch and β-catenin in MDA-MB-231 cells. We also show loss of APT1 depletes the population of CD44+/CD24lo/ALDH+ tumorigenic cells in colony formation assays. Together, the findings of this study demonstrate that palmitoylation, via APT1, is a major mechanism of asymmetric cell division regulating Notch and Wnt-associated protein dynamics, gene expression, and cellular functions.

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Emx2 KO RNAseq

Project description:Emx2 KO RNAseq

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Expression data from Emx2 wildtype and knockout olfactory epithelium

Project description:Emx2 is a homeobox transcription factor that plays a critical role in development. Olfactory sensory neuron axons from Emx2 knockout mice fail to innervate their target tissue, the olfactory bulb. Homeobox transcription factors may also play an important role in olfactory receptor expression. We used microarrays to analyze differences in mRNA abundance in the olfactory epithelium of Emx2 wildtype and knockout embryonic day 18.5 mice. Keywords: Knockout-Wildtype comparision Total RNA from 9 Emx2 wildtype and 9 Emx2 knockout mice was analyzed. Equal amounts of RNA from 3 animals was pooled to create one biological sample, 3 pools per genotype, 6 chips total. RNA was hybridized to the Affymetrix Mouse Exon 1.0 ST array.

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Transcription profiling of mouse Emx2 knock-out urogenital epithelium

Project description:Series of samples studying effect of knock out Emx2 in urogenital epithelium of mouse embryos at E10.5. Experiment Overall Design: The epithelial cells of the gonadal primordium were obtained by Laser Microdissection System. The specimens prepared from three individuals were mixed as one pool. There are three experimental replicates in each genotype, 3-pools of wild-type and 3-pools of Emx2 KO mouse.

2008-06-11 | E-GEOD-10216 | biostudies-arrayexpress

Expression data from Emx2 wildtype and knockout olfactory epithelium

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Emx2 knock-out urogenital epithelium

Project description:Series of samples studying effect of knock out Emx2 in urogenital epithelium of mouse embryos at E10.5. Keywords: repeated sample

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APT1 regulates the asymmetric partitioning of Notch and Wnt signaling during cell division

Project description:APT1 regulates the asymmetric partitioning of Notch and Wnt signaling during cell division

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