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Vitis vinifera

ABSTRACT: Vitis vinifera root miRNA sequencing.

PROVIDER: PRJNA601829 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10906903.fastq.gz	Fastqsanger.gz
	SRR10906904.fastq.gz	Fastqsanger.gz
	SRR10906905.fastq.gz	Fastqsanger.gz
	SRR10906906.fastq.gz	Fastqsanger.gz

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High-throughput sequencing of small RNAs in Vitis vinifera

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High-throughput sequencing of small RNAs in Vitis vinifera

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Project description:Bud endodormancy induction response of two genotypes (Seyval, a hybrid white wine grape and Vitis riparia, PI588259, a native North American grape species) was compared under long (15 h) and short (13 h) photoperiods. Proteins were extracted from both genotypes for all time points and experimental conditions. The proteins were separaed by 2D-PAGE, trypsin digested, and the peptides identified with a MALDI-TOF-TOF mass spectrometer. A master gel was made and mapped with all proteins from both genotypes. The proteins were identified by matching the peptide sequences against the 8X Vitis vinifera grape genome in NCBI. This study was funded by NSF grant DBI064755 and is the result of a collaboration between Dr. Anne Fennell at South Dakota State University and Dr. Grant R. Cramer at the University of Nevada, Reno.

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microRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Vitis vinifera

Project description:Vitis vinifera RNA degradome Isolated polyadenylated RNA from total RNA extracts of Vitis vinifera leaves, were ligated to 5'-adapter that include san MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

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