Project description:Pig brains are valuable models for studying brain diseases due to their structural similarities to human brains. However, understanding the regulatory mechanisms in pig brains is challenging due to cellular heterogeneity. This study aimed to investigate the heterogeneity of pig brains and uncover the underlying regulatory mechanisms at single-cell resolution. We created the first single-cell chromatin accessibility atlas of the cerebral cortex and cerebellum in domestic pigs and wild boars using single-cell ATAC-seq (scATAC-seq). We profiled 71,798 cells, identifying nine cell types, and integrated single-cell RNA sequencing (scRNA-seq) data to characterize cell type-specific regulatory landscapes and oligodendrocyte development. Our analysis revealed that oligodendrocyte progenitor cells exhibit the fastest evolutionary rate between domestic pigs and wild boars. Additionally, cross-species comparison showed that pig regulatory elements are more conserved with humans than those in mice. Notably, genetic variant enrichment studies found that regulatory elements associated with Alzheimer’s disease were significantly enriched in pigs but not in mice, suggesting pigs as a potentially superior model for this condition. Conversely, neurological diseases like schizophrenia and bipolar disorder showed higher enrichment in mice.
2025-05-11 | GSE281568 | GEO
Project description:Microbiome data from rectum and gravesoil of domestic pigs
Project description:The aim of this study was to acquire a better understanding of porcine reproductive and respiratory syndrome (PRRS) disease through a deeper knowledge of gene expression changes that occur in pulmonary lymph nodes by comparing PRRS virus (PRRSV), porcine circovirus type 2 (PCV-2), and swine influenza virus (IAV-S) infections. The PRRSV, IAV-S and PCV-2 viral infections followed a clinical course in these domestic pigs typical of experimental infection of young pigs with these viruses. PRRSV isolate SDSU-73 was pathogenic in this study inducing fever, anorexia, listlessness, and dyspnea.
Project description:African swine fever virus (ASFV) is a highly contagious pathogen that primarily affects domestic and wild pigs with specific tropism to porcine alveolar macrophages (PAMs). However, the host receptors involved in ASFV infection remain unknow. Here, we present a multi-omic epigenetic atlas of ASFV-exposed PAMs and profile 3D chromatin architecture and single-nucleus chromatin accessibility landscapes (sn-ATAC)
2025-04-18 | GSE231435 | GEO
Project description:Rotavirus A in Domestic pigs in Zambia
| PRJNA997783 | ENA
Project description:NERD: oropharyngeal and esophageal microbiome
Project description:Brucella suis infects macrophages and dendritic cells. Wild boars act as reservoirs and carriers of Brucella suis biovar 2, and there is evidence that wild boar can be the main source of infection for domestic pigs through the venereal route. Transmission through this route could be an important path for disesease dissemination. The result from this study will contribute to the overall understanding of the molecular pathogenic mechanisms involved during Brucella suis infection in European wild boar.
Project description:Female domestic pigs were fed a 16-week Lean or Obese diet. Mesenchymal stem/stromal cells (MSCs) were harvested from subcutaneous adipose tissue and expanded for 3-4 passages, and 5hmC profiles were examined through hydroxymethylated DNA immunoprecipitation sequencing (hMeDIP-seq) We hypothesized that obesity and cardiovascular risk factors induce functionally-relevant, locus-specific changes in overall exonic coverage of 5hmC in swine adipose-derived MSCs, and evaluated their reversibility using an epigenetic modulator, vitamin-C.
