Project description:Pacific abalone (Haliotis discus hannai) is one of the most important aquaculture species in East Asia, and growth performance is a key trait for stable and efficient production. To better understand the molecular differences associated with growth variation in this species, we conducted transcriptome profiling of high-growth and low-growth Pacific abalone groups showing significant differences in growth traits. RNA-seq data were generated from hepatopancreas and mantle tissues to identify transcriptional variation associated with growth performance. Comparison of gene expression patterns between the two growth groups provided molecular information relevant to the biological processes and regulatory pathways underlying growth differences in Pacific abalone.
Project description:Temperature profoundly influences the physiology, survival, and distribution of marine ectotherms, including mollusks. Transient receptor potential (TRP) channels are conserved thermosensory proteins in metazoans, yet their evolutionary diversification and functional roles in gastropod mollusks remain unclear. In this study, we present a comprehensive phylogenetic classification and expression analysis of TRP-like channel genes in Pacific abalone (Haliotis discus hannai). Through the extensive mining of genome and transcriptome datasets, we identified 49 TRP-like genes and categorized them into nine families from two major groups: Group 1 (TRPA, TRPC, TRPM, TRPN, TRPS, TRPV, and TRPVL) and Group 2 (TRPP and TRPML), along with two unclassified TRP-like genes. Phylogenetic analysis incorporating sequences from lophotrochozoans, choanoflagellates, fungi, and green algae outlined a lineage-specific TRP-like gene expansion in mollusks. Spatial expression profiling revealed distinct tissue-specific patterns: TRPC-, TRPM-, and TRPP-like genes were enriched in sensory organs (i.e., the eyes and tentacles), whereas TRPM- and TRPV-like genes were expressed predominantly in respiratory and metabolic tissues (i.e., the gills and hepatopancreas). Under acute thermal stress, RNA sequencing and real-time quantitative PCR identified several thermoresponsive TRP paralogs, including TRPA1- and TRPV-like genes, exhibiting distinct transcriptional regulation. These results elucidate the evolutionary complexity and functional diversification of TRP channels in marine gastropods, and highlight the potential role of these molecules in thermal sensing and adaptation. This study provides a molecular framework for understanding TRP-mediated environmental responses in mollusks, contributing to broader insights into marine invertebrate resilience under climate change.
Project description:The circadian rhythm is the most general and important rhythm in biological organisms. In this study, continuous 24 h video recordings showed that the cumulative movement distance and duration of the abalone, Haliotis discus hannai reached their maximum values between 20:00–00:00, but both were significantly lower between 08:00–12:00 than at any other time of day or night (P < 0.05). To investigate the causes of these diel differences in abalone movement behavior, their cerebral ganglia were harvested at 00:00 (group D) and 12:00 (group L) to screen for differentially expressed proteins using tandem mass tagging (TMT) quantitative proteomics. Seventy-five significantly different proteins were identified in group D vs. group L. Acetylcholinesterase (AChE) was found three times, and its expression levels differed significantly between day and night (P < 0.05). A cosine rhythm analysis found that the concentration of acetylcholine (Ach) and the expression levels of AchE tended to be low during the day and high at night, and high during the day and low at night, respectively.
Project description:Lactobacillus plantarum (LPsca12) enhances growth phenotype and muscle nutrition in abalone (Haliotis discus hannai) by modulating (PRJCA041928)
