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Transcriptomics revealed the putative mechanism underlying the impact of HFL1 fibroblasts with overexpression of GALC on LoVo cell

ABSTRACT: Transcriptomics revealed the putative mechanism underlying the impact of HFL1 fibroblasts with overexpression of GALC on LoVo cell

PROVIDER: PRJNA607910 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11130916_1.fastq.gz	Fastqsanger.gz
	SRR11130916_2.fastq.gz	Fastqsanger.gz
	SRR11130917_1.fastq.gz	Fastqsanger.gz
	SRR11130917_2.fastq.gz	Fastqsanger.gz
	SRR11130918_1.fastq.gz	Fastqsanger.gz

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Transcriptomics revealed the putative mechanism underlying the impact of HFL1 fibroblasts with overexpression of GALC on LoVo cell

Project description:Purpose:To elucidate the mechanism by which overexpression of GALC senescent fibroblasts promote the tumor properties of LoVo CRC cells Methods: LoVo cell which co-cultured with LV-GALC HFL1 fibroblasts cells and LoVo cell which co-cultured with LV-NC HFL1 fibroblasts cells using Transwell chamber were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays Conclusions: Our study represents the first detailed analysis of the impact of LV-GALC HFL1 fibroblasts and LV-NC HFL1 fibroblasts on LoVo cell with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles.We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.Fibroblasts regulate the tumorigenicity of CRC cells and play important roles in tumor biology.

2020-02-25 | GSE145662 | GEO

microRNA array of LoVo cells and LoVo-Small Extracellular Vesicles

Project description:LoVo cells were cultured in sEV-depleted (160,000xg, 16h) complete medium and the supernatant were collected after 72h. sEVs were purified and centrifuged at 100,000xg for 2.5h using a Beckman SW41Ti rotor to know the miRNA in LoVo cells and LoVo-Small Extracellular Vesicles

2019-07-01 | GSE117866 | GEO

Gene expression profiling in LoVo-P compared with LoVo-C cells

Project description:The differential expression of mRNA in LoVo-P cells compared with LoVo-C cells was successfully detected using the Arraystar Human LncRNA/mRNAArray V4.0, that used for the global profiling of 20730 human mRNA and 40173 long non-coding RNA (LncRNA) transcripts.

2017-09-20 | GSE103989 | GEO

Expression data from human colorectal cancer cell line LoVo

Project description:PEAK1 overexpression vector and negative control vector were transiently transfected into LoVo cells, respectively, and named LoVo-PEAK1 and LoVo-control groups. LoVo cells of different groups were determined to investigate the different expressions of mRNA using a global gene microarray.

2018-01-10 | GSE108947 | GEO

Expression data of LoVo cells treated by 10mM metformin for eigher 8 or 24h

Project description:Metformin is effective for prevention or treatment of various tumors. There are a lot of studies on the underlying mechanisms of metformin as respect to its anti-tumor action. We used microarrays to detail the global programme of gene expression underlying the anti-tumor effects of metformin on LoVo cells. Human derived LoVo cells were treated with metformin (10mM) for 8 and 24h, and the control and treated cells were harvested for RNA extraction and hybridization on Affymetrix microarrays.The samples were grouped as Met8h, Met24h, Con8h, and Con24h, respectively.

2015-03-31 | E-GEOD-67342 | biostudies-arrayexpress

microRNA array of LoVo cells and LoVo-Small Extracellular Vesicles

Project description:microRNA array of LoVo cells and LoVo-Small Extracellular Vesicles

| PRJNA483459 | ENA

Transcriptomics of LoVo cells exposed to DCC-2036.

Project description:To investigate the mechanisms mediating the inhibitory effects of DCC-2036 on LoVo, gene expression profiling analysis using data obtained from RNA-seq of LoVo cells treated with/without DCC-2036.

2025-01-15 | GSE214063 | GEO

Expression data from Irinotecan resistant LoVo cells comapred to parental LoVo cells

Project description:Irinotecan, an analogue of camptothecin, is frequently used in combination with various anticancer drugs or as a single agent in treatment of colorectal cancer. But drug resistance of tumor is still a major obstacle to overcome for the success of cancer treatment. In this study, We established chronic irinotecan resistant cell line for new marker to increase the sensitivity to irinotecan and investigated gene expression profiles of the irinotecan-resistant colorectal cancer cell line. To create stable CRC cell line chronically resistant to Irinotecan, LoVo cell was exposed to an initial Irinotecan concentration of 0.1 M-NM-<mol/L in RPMI 1640 supplemented with 10% FBS. When the growth of the cultured cells reaches at 80% confluency, cells were passaged twice at same drug concentration to ensure adaptation and then concentration of Irinotecan was sequentially increased in the same manner to 8 M-NM-<mol/L and then we investigated the gene expressions between parental colorectal cancer cell line, LoVo and Irinotecan resistant LoVo cell lines

2014-07-18 | E-GEOD-59501 | biostudies-arrayexpress

Gene expression profiling in LoVo-P compared with LoVo-C cells

Project description:Gene expression profiling in LoVo-P compared with LoVo-C cells

| PRJNA407894 | ENA

Expression data of LoVo cells treated by 10mM metformin for eigher 8 or 24h

2015-03-31 | GSE67342 | GEO