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Transcriptome analysis of tumor infiltrating NK and T cells in CT26-bearing BALB/C mice

ABSTRACT: Transcriptome analysis of tumor infiltrating NK and T cells in CT26-bearing BALB/C mice

PROVIDER: PRJNA608487 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11173859.fastq.gz	Fastqsanger.gz
	SRR11173860.fastq.gz	Fastqsanger.gz
	SRR11173861.fastq.gz	Fastqsanger.gz
	SRR11173862.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of tumor infiltrating NK and T cells in CT26-bearing BALB/C mice

Project description:CT26 tumors were implanted subcutaneously into syngeneic BALB/C mice and allowed to grow for 15-25 days. Tumors were collected, mechanically dissociated, and immune cells enriched using Percoll gradient. Cells were then stained with viability dye, CD45, CD3, and NKp46 to FACS sort for T cells (Live/CD45+/CD3+/NKp46-) and NK cells (Live/CD45+/CD3-/NKp46+). Isolated tumor infiltrating NK and T cells were then processed for RNA sequencing analysis.

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Project description:We reported transcriptional characterization of tumor-infiltrating T regulatory cells (TITRs) that is shared between species and among different tumor types and stages. We genomically profiled immunocytes from 2 species: 1. Mouse: CD4+ Treg and Tconv, and CD8+ T cells from tumor and spleen of B16, MC38 or CT26 tumor bearing mice, as well as from spleens of non-tumor bearing mice. 2. Human: CD4+ Treg cells from surgically resected and cryopreserved human colorectal carcinomas or normal colon tissue.

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