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Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC [RNA-Seq]

ABSTRACT: Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC [RNA-Seq]

PROVIDER: PRJNA609217 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11195133.fastq.gz	Fastqsanger.gz
	SRR11195134.fastq.gz	Fastqsanger.gz
	SRR11195135.fastq.gz	Fastqsanger.gz
	SRR11195136.fastq.gz	Fastqsanger.gz
	SRR11195137.fastq.gz	Fastqsanger.gz

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Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC

Project description:Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC

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Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC [ATAC-Seq]

Project description:Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC [ATAC-Seq]

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Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC [RNA-Seq]

Project description:Endometrial mesenchymal stem cells (eMSC) drive the extraordinary regenerative capacity of the human endometrium. Clinical application of eMSC for therapeutic purposes is hampered by spontaneous differentiation and cellular senescence upon large-scale expansion in vitro. A83-01, a selective TGFbeta receptor inhibitor, promotes pharmacological expansion of eMSC in culture by blocking differentiation and senescence. We subjected primary eMSC treated with A83-01 to RNA sequencing (RNA-seq) to map the underlying gene expression changes. RNA-seq analysis demonstrated that sustained TGFbeta-R inhibition results in differential expression of 1,463 genes.

2020-09-23 | GSE146066 | GEO

Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC [ATAC-Seq]

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2020-09-23 | GSE146065 | GEO

Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-09-23 | GSE146067 | GEO

TGFβ signaling in the uterine epithelium controls endometrial cell homeostasis and regeneration [RNA-Seq]

Project description:In this study, we define the epithelial-specific contribution of SMAD2/3 to endometrial function by conditionally ablating the Smad2 and Smad3 transcription factors using Lactoferrin-iCre (Ltf-cre). Using epithelial organoid cultures from the endometrium, we uncover the signaling mechanisms downstream of TGFβ that control endometrial cell regeneration and are critical for endometrial regeneration and homeostasis.

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TGFβ signaling in the uterine epithelium controls endometrial cell homeostasis and regeneration [CUT&RUN]

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Bone marrow-derived MSC as endometrial stromal progenitor cells

Project description:In the current study, we hypothesized that if bone-marrow derived MSC contribute to endometrial regeneration and are progenitors to hESF, their treatment with agents known to regulate hESF differentiation could promote their differentiation down the stromal fibroblast lineage. To this end, we treated bone marrow-derived MSC with estradiol (E2) and progesterone (P4), BMP2, and activators of the PKA pathway and investigated specific markers of hESF differentiation (decidualization). Furthermore, we investigated the transcriptome of these cells in response to cAMP and compared this to the transcriptome of hESF decidualized in response to activation of the PKA pathway. The data support that MSC can be differentiated down the hESF pathway, as evidenced by changes in cell shape and common expression of decidual markers and other genes important in hESF differentiation and function.

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Transcriptional changes in Wnt antagonism- and TGFβ agonism-induced MSC chondrogenesis.

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Mouse bone marrow Mesenchymal Stem Cells (MSC) exposed to activated T cells

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