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ABSTRACT: RNA-sequencing of bEnd.3 cells

PROVIDER: PRJNA612931 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11318233_1.fastq.gz	Fastqsanger.gz
	SRR11318233_2.fastq.gz	Fastqsanger.gz
	SRR11318234_1.fastq.gz	Fastqsanger.gz
	SRR11318234_2.fastq.gz	Fastqsanger.gz
	SRR11318235_1.fastq.gz	Fastqsanger.gz

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Effect of overexpression of miR-423-5p on gene expression of endothelial cells.

Project description:To investigate machanism of miR-423-5p regulating the angiogenic ability of bEnd.3 cells, we transfected miR-423-5p mimic to overexpress miR-423-5p in bEnd.3 cells. Then we performed high throughput sequencing of miR-423-5p mimic-transfected and control bEnd.3 cells to evaluate different gene expressions between miR-210-3p-overexpressing and control.

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Project description:Dual RNA-sequencing of E.coli and bEnd.3 cells

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Somatic variants of MAP3K3 are sufficient to cause cerebral cavernous malformations

Project description:To further explore the underlying molecular pathways altered by MAP3K3I441M in endothelial cells, the mouse endothelial cell line bEnd.3 was first infected with lentivirus-MAP3K3I441M (LV- MAP3K3I441M-P2A-EGFP; LV-EGFP was used as the control). Total RNA from these cells was then subjected to RNA sequencing.

2023-04-18 | GSE210673 | GEO

The mouse endothelial cell lines

Project description:The bEnd.3 and UV♀2 cells were mouse endothelial cell lines. The bEnd.3 cells were derived from the endothelial cells of the mouse brain and the UV♀2 cells were established from an angioendothelioma-like tumor that developed on the skin of mice. We performed bulk RNA-seq analysis of these two cell lines to investigate the difference between these cells.

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Project description:RNA-seq of seminal plasma exposure of bEND cells

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Effects of StcE treatment on in vitro brain endothelial cell line bEnd.3

Project description:Mucin-domain glycoproteins populate the brain endothelial cell surface, but their roles as a class of glycoconjugates are largely unknown. Here, we perform bulk RNA-sequencing of bEnd.3 cells treated with StcE, which enzymatically degrades cell surface mucins, in order to understand the effects of mucin removal on brain endothelial cell function.

2024-12-17 | GSE268263 | GEO

Transcription profile of intracellular H37Ra of bEnd.3 and Raw 264.7 cells

Project description:Transcription profile of intracellular H37Ra of bEnd.3 and Raw 264.7 cells 3 d.p.i

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RNA-seq analysis of NDP and F4L5.13 treated bEnd.3 endothelial cell line

Project description:bEnd.3 cells were treated with NDP (Norrin) or F4L5.13 (FZD4-LRP5 agonist) for 8 or 24 hours

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Transcriptome profile of M.tb treated/untreated host cells

Project description:Transcriptome profile of M.tb uninfected or infected 0 d.p.i or 3 d.p.i Raw264.7, bEnd.3, THP1, A549, hBMEC cells

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Identification of the molecular basis of anti-cancer effect of Huaier.

Project description:Intervention type:DRUG. Intervention1:Huaier, Dose form:GRANULES, Route of administration:ORAL, intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary. Control intervention1:None. Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes. For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.. Timepoint:RNA sequencing of 240 blood samples of 80 cases and its analysis, scheduled from June 30, 2022..

| 2612481 | ecrin-mdr-crc

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