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MiCLIP-seq of postnatal day 0 (P0) normal mouse skin epithelial cells

ABSTRACT: miCLIP-seq of postnatal day 0 (P0) normal mouse skin epithelial cells

PROVIDER: PRJNA614972 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11409411_1.fastq.gz	Fastqsanger.gz
	SRR11409411_2.fastq.gz	Fastqsanger.gz
	SRR11409412_1.fastq.gz	Fastqsanger.gz
	SRR11409412_2.fastq.gz	Fastqsanger.gz
	SRR11409413_1.fastq.gz	Fastqsanger.gz

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miCLIP-seq of postnatal day 0 (P0) normal mouse skin epithelial cells

Project description:We employed miCLIP-seq to profile the location and extent of m6A in the Poly(A)+ transcriptome. Skin epithelial cells were isolated from wild-type P0 neonates by FACS. Total RNA was extracted by TRIzol-LS and Poly(A)+ RNA was extracted with Dynabeads™ mRNA Purification Kit (Thermo-Fisher, 61006). Input and miCLIP libraries was prepared from 3 biological replicates with each containing RNA isolated from 3 litters of neonates. Libraries were sequenced on the Illumina Hi-seq platform to generate paired-ended 50 bp reads. Sequencing data was processed as described in (Geula et al., 2015).

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Project description:The goal of the study was to compare gene expression of P0 wild-type, P0 Fezf2-/- cortices, and Fezf2-/-; Fezf2-EnR cortices. Total RNAs were isolated from P0 cortices dissected from wild-type (n=3), Fezf2-/- mice (n=4), and Fezf2-/-; Fezf2-EnR cortices (n=2), following Qiagen RNAeasy kit instruction.Sequence libraries were made following Illumina RNA PolyA library preparation guide.The libaries were pair-end sequenced (150nt per end). Differentially expressed genes were identified by DESEQ.

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