Rattus norvegicus albus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA616428 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Molecular alterations in areas generating fast ripples in an animal model of temporal lobe epilepsy
| PRJNA282722 | ENA
Project description:Rat model of MTLE: Animals with epilepsy vs animals without epilepsy (codelink)
| PRJNA142077 | ENA
Project description:Rat model of MTLE: Animals with epilepsy vs animals without epilepsy (Agilent)
| PRJNA141935 | ENA
Project description:Gene Expression Analysis in an Animal Model of Cortical Dysplasia-CONTROL GROUP
| PRJNA110423 | ENA
Project description:Gene Expression Analysis in an Animal Model of Cortical Dysplasia-IRRADIATED GROUP
| PRJNA109421 | ENA
Project description:Expression Profiling of a Genetic Animal Model of Depression
| PRJNA125511 | ENA
Project description:Global analysis of genes regulated by canonical Wnt pathway
| PRJNA306922 | ENA
Project description:Knee osteoarthritis (KOA), as a degenerative multifactorial disease, affects the quality of life and mental health of patients, and also brings a huge socioeconomic burden. Treating synovitis have shown promise as anti-inflammatory therapeutics in mitigating OA symptoms and disease progression. Here, by analysing synovial single-cell sequencing (scRNA-seq) data from KOA, we found that synovial fibroblasts (FLS) in OA synovium showed a distinct pro-inflammatory phenotype. We collected synovial tissue from patients with clinical OA as well as from healthy donors, and histological examination was consistent with findings in scRNA-seq. Inspired by recent cross-tissue fibroblast lineage studies, we identified by sequencing that healthy FLS in synovial tissues share transcriptome-level similarities with dermal fibroblasts (DFb). Subsequently, we revealed the local as well as systemic distribution of intra-articular injected DFbs by constructing/extracting two types of rat fibroblasts (luciferase DFbs as well as GFP DFbs). The results demonstrate that DFbs can be locally retained in the synovium for up to three weeks following targeted engrafting on it. And intra-articular injection does not result in DFbs migration to vital organs or the occurrence of histological changes in these organs. A rat model of KOA was constructed by anterior cruciate ligament transection (ACLT) in order to study the therapeutic effect of DFbs on KOA. After injection, the rats showed improvement in painful gait. In addition, histological as well as imaging results showed reduced synovitis and improvement in articular cartilage. Finally we verified the protective effect of DFbs on cytokine-stimulated chondrocytes in a co-culture system.
2026-06-24 | GSE304724 | GEO
Project description:We analyzed the effects of antibiotics using a popular model of gut microbiota depletion in mice by a cocktail of antibiotics. We combined intestinal transcriptome together with metagenomic analysis of the gut microbiota to develop a new bioinformatics approach that probes the links between microbial components and host functions. We found that most antibiotic-induced alterations can be explained by three factors: depletion of the microbiota; direct effects of antibiotics on host tissues; and the effects of remaining antibiotic-resistant microbes. While microbe depletion led to down-regulation of immunity, the two other factors primarily inhibited mitochondrial gene expression and amounts of active mitochondria, and induced cell death. By reconstructing and analyzing a transkingdom network, we discovered that these toxic effects were mediated by virulence/quorum sensing in antibiotic-resistant bacteria. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
2015-01-26 | E-GEOD-60568 | biostudies-arrayexpress