Project description:fungal Exploring the effects of volcanic eruption disturbances on soil microbial communities in the montane meadow steppe Genome sequencing
Project description:Land cover change has long been recognized that marked effect the amount of soil organic carbon. However, little is known about microbial-mediated effect processes and mechanism on soil organic carbon. In this study, the soil samples in a degenerated succession from alpine meadow to alpine steppe meadow in Qinghai-Tibetan Plateau degenerated, were analyzed by using GeoChip functional gene arrays.
Project description:The aim of this study was to evaluate and compare the gene expression profiles of dental follicle and periodontal ligament in humans, which can possibly explain their functions of dental follicle and PDL such as eruption coordination and stress resorption. That may apply this information to clinical problem like eruption disturbance and to periodontal tissue engineering. PDL samples were obtained from permanent premolars (n=11) and dental follicle samples were obtained during extraction of supernumerary teeth (n=4). Comparative cDNA microarray analysis revealed several differences in gene expression between permanent PDL and dental follicles.
Project description:Background: Probiotic-like bacteria treatment has been described to be associated with gut microbiota modifications. Goal: To decipher if the effects of the tested probiotic-like bacteria are due to the bacteria itself or due to the effects of the bacteria on the gut microbiota. Methodology: In this study, gut microbiota has been analyzed from feces samples of subjects with metabolic syndrome and treated with one of the 2 tested probiotic-like bacteria or with the placebo during 3months.
Project description:In search of markers for protein aging, we used an extraordinary test case of human bones from the excavations sites of Pompei and Herculaneum (79 AD), analyzed by a shotgun proteomic approach using LC-MS/MS. Human bones from Scalandrone Bay (II sec AD, another volcanic area, yet not related to Vesuvius eruption were also analysed for the comparison of the PTMs provoked from a volcanic ground to an almost coeval standard burial condition. In addition, already published archaeological bones, approximately 17 centuries younger than the test case, were used as control samples. Deamidation of asparagine and glutamine was examined as a now widely considered aging molecular marker. Deamidation profile was quite robust for samples with a similar age whereas the fewer proteins were detected, the more degraded the sample were found to be. Positional investigation of deamidation in the polypeptide sequence of collagen type I revealed that some zones are more susceptible to deamidation than others. Back bone cleavage analysis confirmed that some zones apart from deamidation are more susceptible to spontaneous hydrolysis too. Analysis of post-translational modifications showed higher advanced glycation products of lysine and arginines in the samples of Pompeii and Herculaneum. Furthermore, oxidative products of methionine, histidine and the conversion of ST to glycine were also detected. Interestingly, multiple oxidation products of proline and dehydration of hydroxyproline were detected for the first time, turning the scientific interest to this amino acid (P), which is usually ignored due to its natural conversion to hydroxyproline. As a matter of fact, we can observe that collagen degradation in bones from Pompeii and Herculaneum underwent different degradation processes.
Project description:The aim of this study was to evaluate and compare the gene expression profiles of dental follicle and periodontal ligament in humans, which can possibly explain their functions of dental follicle and PDL such as eruption coordination and stress resorption. That may apply this information to clinical problem like eruption disturbance and to periodontal tissue engineering.
