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ABSTRACT: Effect of calcium on grape berry cracking

PROVIDER: PRJNA624905 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR11531447_1.fastq.gz	Fastqsanger.gz
	SRR11531447_2.fastq.gz	Fastqsanger.gz
	SRR11531448_1.fastq.gz	Fastqsanger.gz
	SRR11531448_2.fastq.gz	Fastqsanger.gz
	SRR11531449_1.fastq.gz	Fastqsanger.gz

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Grape berry developmental series comparing pruning treatments from a vineyard in Clare Valley, South Australia (CLA-05)

Project description:Changes in gene expression during berry development during a grape growing season were analysed. The effect on gene expression of different viticultural practises during grape berry development was investigated in this study by comparing two pruning methods (spur versus machine). Grape berries were collected and pooled on a weekly basis to obtain a developmental series comprised of 17 developmental stages from flowering until harvest across the grape growing season for both spur and machine pruned vines. Gene expression patterns during development and between pruning treatments were obtained. Keywords: Time course, developmental series and treatments

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Project description:Changes in gene expression during berry development during a grape growing season were analysed. The effect on gene expression of different viticultural practises during grape berry development was investigated in this study by comparing two irrigation methods (standard versus prolonged deficit irrigation). Grape berries were collected and pooled on a weekly basis to obtaining a developmental series comprising of 17 developmental stages from flowering until harvest across the grape growing season for both standard and prolonged deficit irrigated vines. Gene expression patterns during development and between pruning treatments were obtained. Keywords: Time course, developmental series and treatments

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Project description:Transcriptomic analysis of grape berry in response to calcium

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grape berry vacuole proteomic - Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins

Project description:The vacuole occupies a large portion of plant cell volume, it is especially true to fruit tissues. Berry flesh cell vacuole serves as storage organelle for water, sugars, acids, secondary metabolites and others, which largely determining berry quality (Fontes et al., 2011a, b; Shiratake and Martinoia, 2007, Conde et al., 2006). However, the molecular basis of these compartmentation processes is still poorly understood. As in many species, the major bottle neck to study these aspects in grapevine is to obtain highly purified vacuoles with a good yield (Fontes et al., 2010). Up to date, several vacuole or tonoplast proteome researches were applied on a few plants mainly on Arabidopsis thaliana, vacuoles or tonoplast were derived from mesophyll cells (Carter et al., 2004, Endler et al., 2006, Schulze, et al., 2012) or cell culture (Jaquinod et al 2006, Shimaoka et al 2004), cauliflower buds (Schmidt et al., 2007) and sugar beet taproots (Jung et al., 2015). Though the grape berry protoplasts and intact vacuoles were successfully isolated from Cabernet Sauvignon berry suspension-cultured cells (Fontes et al., 2010), the vacuoles isolated from grape berry or different development and ripening stages of grape berry mesocarp tissues were not achieved.

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