Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of needling treatments on GB20 acupoint in EAE model mice

ABSTRACT: Effect of needling treatments on GB20 acupoint in EAE model mice

PROVIDER: PRJNA625656 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11551934_1.fastq.gz	Fastqsanger.gz
	SRR11551934_2.fastq.gz	Fastqsanger.gz
	SRR11551935_1.fastq.gz	Fastqsanger.gz
	SRR11551935_2.fastq.gz	Fastqsanger.gz
	SRR11551936_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Effect of needling treatments on GB20 acupoint in EAE model mice

Project description:Purpose: We used RNA sequencing to investigate the effect of needling treatments on GB20 acupoint in EAE model mice Methods: Retinal mRNA profiles of 13-week-old female sham control mice (SHAM), EAE model mice with MOG injection (MOG), EAE model mice with needling treatment at acupoint GB20 (GB20) and its control acupoint GV16 (GV16) were generated by deep sequencing, in triplicate, using Illumina Hiseq platform. The sequence reads that passed quality filters were analyzed with DESeq2 R package to identify differently expressed genes with padj<0.05. Results: RNA-seq identified total 234 DEGs, with 100 genes upregulated and 134 genes downregulated, comparing MOG group with SHAM group.Further analysis revealed that, among the MOG injection induced 100 up-regulated DEGs, 24 DEGs were reversed and significantly down-regulated by GB20 treatment, while 14 were reversed and significantly down-regulated by GV16 treatment. However, 12 of the 14 reversed DEGs of GV16 treatment were already included in the 24 DEGs of GB20. The left 2 DEGs specific to GV16 treatment were Gfap (glial fibrillary acidic protein) and Fgf2 (fibroblast growth factor 2). As to the MOG injection induced 134 down-regulated DEGs, 12 DEGs were reversed and significantly up-regulated by GB20 treatment, while only 3 DEGs were reversed and significantly up-regulated by GV16 control treatment, and all the 3 DEGs were included in the 12 DEGs of GB20 treatment. Conclusions: Our research first reported that needling treatment on GB20 had regulation effects on retinal transcriptome of EAE model mice, indicating potential way of therapy for treatment of optic neuritis.

2020-10-27 | GSE148759 | GEO

Spinal cord gene expression profiling of the effect of EHP-101 in EAE model

Project description:Spinal cord gene expression profiling of the effect of EHP-101 in EAE model

2020-06-21 | GSE131854 | GEO

Effect of Clostridia strains on EAE clinical outcome as a therapeutic treatment

Project description:Researchers have induced an experimental model of multiple sclerosis [experimental autoimmune encephalomyelitis (EAE)] in mice to investigate the therapeutic effect of the oral treatment with selected Clostridia strains on the clinical outcome of EAE and its mechanisms of action

2021-09-15 | GSE155438 | GEO

Mouse brain proteome map of Experimental autoimmune encephalomyelitis (EAE) mouse model

Project description:Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS); its cause is unknown. To understand the pathogenesis of MS, researchers often use the experimental autoimmune encephalomyelitis (EAE) mouse model. Here, our aim was to build a proteome map of the biological changes that occur during MS at the major onset sites—the brain and the spinal cord. We performed quantitative proteome profiling in five specific brain regions and the spinal cord of EAE and healthy mice with high-resolution mass spectrometry based on tandem mass tags.

2019-02-25 | PXD011428 | Pride

Effect of acupuncture treatments on retinal transcriptome after optic nerve injury

Project description:Purpose: We used RNA sequencing to investigate the effect of acupuncture treatment on retinal transcritome after optic nerve injury Methods: Retinal mRNA profiles of 5-week-old wild-type (WT), optic nerve crush injury (ONC), ONC injury with acupuncture treatment at acupoint GB20 (ONC-F) and ONC injury with acupuncture treatment at acupoint BL1 (ONC-J) mice were generated by deep sequencing, in triplicate, using Illumina Hiseq platform. The sequence reads that passed quality filters were analyzed with DESeq2 R package to identify differently expressed genes with padj<0.05. qRT–PCR validation was performed using TaqMan and SYBR Green assays Results: RNA-seq revealed that 436 genes including 31 transcription factors (TFs) were changed after injury, which include many well-known neural degeneration related TFs such as Jun, Ddit3, Atf3 and Atf4. Interestingly, acupuncture treatment at acupoint GB20 (Fengchi) or BL1(Jingming) after ONC injury both resulted in change of retinal gene expression, compared with ONC injury without treatment; furthermore, acupuncture treatment also reversed several gene expression status induced by axon injury. Conclusions: Our research first reported that acupuncture treatment after injury can regulated retinal transcriptome and reversed the gene expression level induced after injury, which will provide novel therapeutic targets for treatment of retinopathy diseases.

2019-11-12 | GSE131486 | GEO

Spinal cord gene expression profiling of the effect of EHP-101 in EAE model

Project description:Spinal cord gene expression profiling of the effect of EHP-101 in EAE model

| PRJNA545128 | ENA

Gene expression profiling of mouse experimental autoimmune encephalomyelitis (EAE) from spinal cord preparations of opticospinal EAE (OSE) and MOG-induced EAE

Project description:Genetic opticospinal EAE (OSE) and MOG-induced EAE (MOG-EAE) are two experimental autoimmune encephalomyelitis (EAE) mouse models of human multiple sclerosis. For the OSE model, double-transgenic 2D2 (TCRMOG) x IgHMOG mice were used. For MOG-EAE, wildtype C57BL/6 mice were immunized with a MOG peptide consisting of the amino acids 35-55, administered in complete Freund’s adjuvant containing 5mg / ml Mycobacterium tuberculosi. The severity of EAE was rated on the scale 0: healthy animal; 1: animal with a flaccid tail; [...]; 4: animal with both hind legs paralyzed. The case groups in the experiment were: OSE1: OSE with disease score 1; OSE4: OSE with disease score 4; MOG4: MOG-EAE injected with both MOG and adjuvant, with disease score 4. The control groups in the experiment were: OSE0: OSE with disease score 0; CFA: C57BL/6 mice injected only with adjuvant (no MOG); WT: Wildtype C57BL/6 mice. The aim of the experiment was to assess gene expression differences 1) between OSE4 and OSE0, 2) between OSE1 and OSE0, and 3) between MOG4 and CFA. For control, WT was compared to OSE0 and CFA. Subsequently, differentially expressed transcripts were compared, first, between the OSE4 vs. OSE0 and the MOG4 vs. CFA contrasts (different EAE models) and, second, between the OSE4 vs. OSE0 and the OSE1 vs. OSE0 contrasts (different EAE severity).

2020-08-21 | E-MTAB-9132 | biostudies-arrayexpress

Effect of ORY-2001, ORY-LSD1, FTY720 on gene expression in EAE mice.

Project description:Gene expression analysis in the spinal cord and brain of vehicle-treated and ORY-2001 and ORY-LSD1 treated EAE mice in the subchronic (SC) and of ORY-2001 and ORY-LSD1 in the effector (E) phase in EAE mice

2021-08-03 | GSE118071 | GEO

Stool proteome of EAE mice prior to physical symtoms but after immunization

Project description:TMT-labeled shotgun proteomics of mouse stool collected before and after EAE immununization.

2019-11-13 | PXD012287 | Pride

Transcriptome profiling of a backcross between experimental autoimmune encephalomyelitis (EAE)-susceptible DA and EAE-resistant PVG rat strains to identify expression quantitative trait loci.

Project description:The aim of the experiment is to characterize the genetics and mechanisms of the inflammatory response after induction of experimental autoimmune encephalomyelitis (EAE), a model of multiple sclerosis, by studying the genome wide expression in the spleen in late disease. A backcross was created between EAE-susceptible DA and EAE-resistant PVG rats. At day 35 after induction of EAE with myelin oligodendrocyte glycoprotein (MOG) in these rats, spleens were taken for transcriptional profiling.

2013-08-10 | E-MTAB-784 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data