Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells Provide Insights on Parenteral Nutrition Associated Cholestasis in the Immature Liver

ABSTRACT: Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells Provide Insights on Parenteral Nutrition Associated Cholestasis in the Immature Liver

PROVIDER: PRJNA625801 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11555001.fastq.gz	Fastqsanger.gz
	SRR11555002.fastq.gz	Fastqsanger.gz
	SRR11555003.fastq.gz	Fastqsanger.gz
	SRR11555004.fastq.gz	Fastqsanger.gz
	SRR11555005.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 28

Similar Datasets

Transcriptomic analysis of early IFALD in preterm piglets receiving parenteral nutrition

Project description:Preterm Yorkshire piglets were provided parenteral nutrition for 14 days and developed cholestasis and histologic liver injury. Bottle-fed age-matched controls were provided sow milk replacer every 2-3 hours for 14 days.

2023-08-02 | GSE234108 | GEO

Transcriptomic analysis of SEFA-6179 treatment in preterm piglets receiving parenteral nutrition

Project description:Preterm Yorkshire piglets were provided parenteral nutrition for 14 days, receiving treatment with SEFA-6179 or a medium chain triglyceride vehicle control. At the end of 14 days, MCT piglets developed cholestasis and histologic liver injury.

2023-06-03 | GSE223347 | GEO

Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells Provide Insights on Parenteral Nutrition Associated Cholestasis in the Immature Liver

Project description:Our findings provide insights into altered bile acid and lipid metabolizing processes in the immature human liver and support the use of iHLC as a relevant in vitro system for modeling the developing liver to study lipid metabolism and PNAC.

2021-04-15 | GSE148790 | GEO

RNA-sequencing identifies novel transcriptomic signatures in parenteral nutrition-associated liver disease

Project description:RNA-sequencing in liver from control and parenteral nutrition-associated liver disease (PNALD) rats

2022-06-01 | GSE171007 | GEO

Altered RNA expression in parenteral nutrition mouse model

Project description:We reported altered RNA expression profiles in wild-type (WT) C57BL/6J mice upon parenteral nutrition treatment compared to saline infusion controls. RNA samples used were isolated from the livers of PN or saline infused mice. RNA samples from 3 saline or 3 PN mouse livers were pooled, respectively. Final fold change were calculated by normalize the signal intensity of each probe in PN sample to that of the saline sample.

2016-02-01 | GSE71286 | GEO

Multicomponent Parenteral Lipid Emulsions Do Not Protect Against Liver Injury in Neonatal Pigs with Obstructive Cholestasis

Project description:Biliary atresia (BA) is a pediatric liver disease that often necessitates parenteral nutrition (PN) to support growth due to impaired liver function. While soy oil lipid emulsions (SLE) are commonly used in PN, they may contribute to cholestatic liver injury. In contrast, mixed oil lipid emulsions (MLE) have shown promise in preventing cholestasis in non-BA infants, potentially by restoring bile flow. However, their effectiveness in cases of complete bile duct obstruction, as seen in BA, remains uncertain. To explore the potential benefits of MLE in BA, we utilized a neonatal pig model of bile duct ligation (BDL). Pigs underwent either BDL or sham surgery and were subsequently fed either MLE or SLE via PN, or enterally with formula. The MLE-BDL pigs exhibited significantly greater weight gain compared to those fed SLE or formula enterally. Additionally, MLE-BDL pigs showed higher serum bile acid and gamma-glutamyl transferase concentrations compared to SLE-BDL pigs. However, no significant differences in liver injury, assessed by ductular reaction or fibrosis, were observed between MLE- and SLE-BDL pigs. Based on weight gain alone, MLE may be a superior lipid emulsion for use in neonates with obstructive cholestasis.

2025-06-16 | GSE281589 | GEO

Investigation of parenteral nutrition-induced hepatotoxicity using human liver spheroid co-cultures

Project description:Parenteral nutrition (PN) is typically administered to individuals with gastrointestinal dysfunction, a contraindication for enteral feeding and a need for nutritional therapy. When PN is the only energy source in patients, it is defined as total parenteral nutrition (TPN). TPN is a life-saving approach for different patient populations, both in infants and adults. However, despite numerous benefits, TPN can cause adverse effects, including metabolic disorders and liver injury. TPN-associated liver injury, known as intestinal failure-associated liver disease (IFALD), represents a significant problem affecting up to 90% of individuals receiving TPN. IFALD pathogenesis is complex, depending on the TPN components as well as on the patient’s medical conditions. Despite numerous animal studies and clinical observations, the molecular mechanisms driving IFALD remain largely unknown. The present study was set up to elucidate the mechanisms underlying IFALD. For this purpose, human liver spheroid co-cultures were treated with TPN mixture followed by RNA sequencing analysis. It was found that prolonged exposure to TPN substantially changes the transcriptome profile of liver spheroids and affects multiple metabolic and signaling pathways contributing to liver injury.

2024-05-03 | GSE264357 | GEO

Human induced pluripotent stem cell derived hepatocytes provide insights on parenteral nutrition associated cholestasis in the immature liver.

Project description:Parenteral nutrition-associated cholestasis (PNAC) significantly limits the safety of intravenous parenteral nutrition (PN). Critically ill infants are highly vulnerable to PNAC-related morbidity and mortality, however the impact of hepatic immaturity on PNAC is poorly understood. We examined developmental differences between fetal/infant and adult livers, and used human induced pluripotent stem cell-derived hepatocyte-like cells (iHLC) to gain insights into the contribution of development to altered sterol metabolism and PNAC. We used RNA-sequencing and computational techniques to compare gene expression patterns in human fetal/infant livers, adult liver, and iHLC. We identified distinct gene expression profiles between the human feta/infant livers compared to adult liver, and close resemblance of iHLC to human developing livers. Compared to adult, both developing livers and iHLC had significant downregulation of xenobiotic, bile acid, and fatty acid metabolism; and lower expression of the sterol metabolizing gene ABCG8. When challenged with stigmasterol, a plant sterol found in intravenous soy lipids, lipid accumulation was significantly higher in iHLC compared to adult-derived HepG2 cells. Our findings provide insights into altered bile acid and lipid metabolizing processes in the immature human liver, and support the use of iHLC as a relevant model system of developing liver to study lipid metabolism and PNAC.

| S-EPMC8196029 | biostudies-literature

Transcriptomic analysis of early IFALD in preterm piglets receiving parenteral nutrition

Project description:Transcriptomic analysis of early IFALD in preterm piglets receiving parenteral nutrition

| PRJNA980120 | ENA

Investigation of parenteral nutrition-induced hepatotoxicity using human liver spheroid co-cultures

Project description:Investigation of parenteral nutrition-induced hepatotoxicity using human liver spheroid co-cultures

| PRJNA1102022 | ENA