Project description:The purpose of this study was to determine the difference of the miRNA profiles between normal and preeclamptic placenta. Ten placental samples were analyzed. Six were from preeclamptic patients and four were from normal pregnancies.
Project description:Plasma from normal and preeclamptic pregnancies was collected and added in the culture medium of HUVEC cells. The aims are double: identifying putative biomarkers that could be modified recurrently in the endothelium under preeclampsia and understand at term the molecular bases of the increased risk of cardiovascular and kidney diseases in woman that have had a preeclampsia. 2 pools of 3 control plasmas and 2 pools of 4 preeclamptic plasma were organized and added at a concentration of 1/10 on HUVEC cells, during 24 hours before transcriptomic analysis
Project description:Plasma from normal and preeclamptic pregnancies was collected and added in the culture medium of HUVEC cells. The aims are double: identifying putative biomarkers that could be modified recurrently in the endothelium under preeclampsia and understand at term the molecular bases of the increased risk of cardiovascular and kidney diseases in woman that have had a preeclampsia.
Project description:Genome wide DNA methylation profiling of normal and preeclampsia placental samples. Illumina Infinium HumanMethylation450 BeadChip (450K array) was used to obtain DNA methylation profiles in placental samples. Samples included 16 samples from healthy uncomplicated pregnancies and 8 samples from pregnancies affected by preeclampsia.
Project description:Plasma from pregnant women were applied three days in three steps to HUVEC cells. RNAs were isolated and prepared. Four pools of RNAs were obtained, two of them from cells exposed to preeclamptic plasma (2 and 3, corresponding to mild and severe preeclampsia, respectively) and two from cells exposed to normal plasma from pregnant women (five and five).