Project description:Total RNA isolated from maternal serum (drawn between 36-37 weeks gestation)from pregnancies with a growth restricted infant defined as an Individualised birthweight centile (IBC) < 3rd matched to a control pregnancy (IBC 20-80th), n=4 per group with 2 female and 2 male infants per group. Maternal serum miRNAs profiled using miRCURY LNA Universal RT miRNA PCR Human panel I + II to determine biomarker potential for prediction of growth restriction and sexually dimorphic patterns.
Project description:Mass spectrometry-based proteomics has greatly advanced our understanding of biological processes across diverse fields, including disease and oncology. Despite this progress, the maternal-fetal interface, particularly in the context of fetal aneuploidy, remains underexplored. This study addressed this gap by performing an exploratory, label-free mass spectrometry-based proteomic analysis of maternal urine from 15 pregnancies with normal karyo-type and 9 with fetal aneuploidy.