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Gene expression profile in human cumulus cells (CCs) during long-term in vitro culture

ABSTRACT: Gene expression profile in human cumulus cells (CCs) during long-term in vitro culture

PROVIDER: PRJNA627191 | ENA |

REPOSITORIES: ENA

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Gene expression profile in human cumulus cells (CCs) during long-term in vitro culture

Project description:In the ovarian follicle, maturation of the oocyte increases in the presence of somatic cells called cumulus cells (CCs). These cells form a direct barrier between the oocyte and external environment. Thanks to bidirectional communication, they have a direct impact on the oocyte, its quality and development potential. Understanding the genetic profile of CCs appears to be important in elucidating the physiology of oocytes. In this work, CCs were subjected to in vitro long-term culture. RNA was collected after 1, 7, 15 and 30 days of culture. Expression microarrays were used for analysis, which allowed to identify groups of genes characteristic for particular cellular processes. In this study, we demonstrated the gene expression profile of long time primary cultured human cumulus cells.

2020-04-22 | GSE149033 | GEO

Epigenetic drift during long-term culture of cells in vitro

Project description:Epigenetic drift during long-term culture of cells in vitro

| PRJNA478324 | ENA

Epigenetic drift during long-term culture of cells in vitro

Project description:Epigenetic drift during long-term culture of cells in vitro

| PRJNA603001 | ENA

Gene expression profile in human ovarian granulosa cells during long-term in vitro culture

Project description:Granulosa cells (GCs) have many endocrine functions. However, in long-term in vitro culture GCs can change their properties. GCs were collected from hyper-stimulated ovarian follicles from woman during IVF procedures. They were cultured in in vitro long-term culture. RNA was collected after 1, 7, 15 and 30 days of culture. Expression microarrays were used for analysis, which allowed to identify groups of genes characteristic for particular cellular processes. In this study, we demonstrated the gene expression profile of long time primary cultured human ovarian granulosa cells.

2019-04-17 | GSE129919 | GEO

Effect of adenosine on the gene expression profile of cumulus cells in vitro cultured as cumulus-oocyte complexes for 6 h

Project description:Changes in gene expression induced by adenosine treatment in cumulus cells using Agilent EmbryoGENE microarray slides. Bovine cumulus-oocyte complexes in vitro-cultured for 6 h using adenosine and compared to non-treated (control). After culture, COC were were washed and cumulus cells were used for microarray analysis

2018-09-12 | GSE119804 | GEO

Epigenetic drift during long-term culture of cells in vitro [4C]

Project description:Epigenetic drift during long-term culture of cells in vitro [4C]

| PRJNA695204 | ENA

Long-term culture of alveolospheres

Project description:Long-term culture of alveolospheres

| PRJNA573090 | ENA

Effect of FSH on the gene expression profile of cumulus cells in vitro cultured as cumulus-oocyte complexes in presence of adenosine for 6 h

Project description:Changes in gene expression induced by FSH in cumulus cells treated with adenosine using Agilent EmbryoGENE microarray slides. Bovine cumulus-oocyte complexes in vitro-cultured for 6 h using adenosine. The effect of FSH was compared with control as non-treated cumulus-oocyte complexes. After culture, COC were were washed and cumulus cells were used for microarray analysis

2018-09-12 | GSE119803 | GEO

Expression profiling and function analysis identified new cumulus cells-expressed genes and miRNAs predictive of oocyte developmental potential

Project description:Although studies have identified cumulus cells (CCs)-expressed genes/miRNAs that regulate cumulus expansion and/or CC apoptosis and may be used as markers for selection of competent oocytes/embryos, CCs-expressed genes/miRNAs whose expression levels are directly correlated with oocyte developmental potential (DP) are urgently needed. To identify CCs-expressed DP-directly-related genes/miRNAs, we first established CC models from mouse cumulus-oocyte-complexes that showed significantly different DP after maturation in vitro or in vivo. By conducting mRNA/miRNA sequencing and functional analysis using such in vitro and in vivo CC models, we identified and validated new sets of CCs-expressed genes/miRNAs, whose expression levels were directly correlated with oocyte DP. Thus, we identified and validated Spp1, Fn1, Sdc1 and Ngf as DP-beneficial genes, Fos and Jun as DP-detrimental genes, and miR-7686-5p, miR-133a-3p, novel-miR-239, novel-miR-193 and miR-339-5p as DP-detrimental miRNAs. Furthermore, the close similarities in top KEGG pathways among 4 different experiments suggest that CCs-expressed genes and/or miRNAs regulate oocyte DP mostly indirectly through regulating cumulus expansion and/or CC apoptosis. The new genes/miRNAs identified can be used as markers for selection of competent oocytes/embryos, and the data will contribute to elucidating oocyte maturation mechanisms.

2025-06-18 | GSE291430 | GEO

Human cumulus cells

Project description:Cumulus cells (CCs) are biologically distinct from other follicular cells and perform specialized roles, transmitting signals within the ovary and supporting oocyte maturation during follicular development. The Affymetrix 3’ IVT express protocol was used to prepare cRNA (one-cycle amplification) with a starting concentration of 100 ng of total RNA To compare groups of CCs at different stages of oocyte nuclear maturation, a Significance Analysis of Microarrays (SAM) was performed.

2012-03-10 | GSE31681 | GEO

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