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RNA-Seq of human fetal and adult scalp hair follicle dermal papilla (DP), dermal sheath cup (DSC), and interfollicular dermal (IFD) cells

ABSTRACT: RNA-Seq of human fetal and adult scalp hair follicle dermal papilla (DP), dermal sheath cup (DSC), and interfollicular dermal (IFD) cells

PROVIDER: PRJNA627617 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11595760.fastq.gz	Fastqsanger.gz
	SRR11595761.fastq.gz	Fastqsanger.gz
	SRR11595762.fastq.gz	Fastqsanger.gz
	SRR11595763.fastq.gz	Fastqsanger.gz
	SRR11595764.fastq.gz	Fastqsanger.gz

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RNA-Seq of human fetal and adult scalp hair follicle dermal papilla (DP), dermal sheath cup (DSC), and interfollicular dermal (IFD) cells

Project description:Much is still unknown about the molecular regulatory networks which govern the dermal papilla’s (DP) ability to induce hair follicle regeneration, a capacity which gradually decreases with age. DP and dermal sheath cup (DSC) cells from mature, anagen phase, hair follicles were manually microdissected from fresh frozen sections of 16-18 week human fetal scalp and 30-60 year old adult male scalp. Interfollicular dermal (IFD) fibroblasts were harvested for comparison. RNA-seq libraries from each cell population were prepared with Nugen’s Ovation RNA-Seq and Ultralow Library systems, sequenced to approximately 100 million total reads. A set of 121 genes was identified as significantly upregulated in fetal DP cells, as compared to both fetal DSC and IFD populations. Wnt/β-catenin, Shh, FGF, BMP, and Notch signaling pathways were significantly enriched among those genes differentially expressed between fetal and adult DP cells. Among them, Spondin-1, a Wnt agonist, was chosen for verification and can rescue hair follicle regeneration in skin reconstitution assays using cells from adult mice. Additionally, twenty-nine transcription factors were significantly upregulated in fetal DP compared to adult DP cells. Of those, seven transcription factor binding motifs were significantly enriched in the candidate promoter regions of differentially expressed genes between fetal and adult DP cells, suggesting a combinatorial regulatory role in the fetal DP phenotype. Further investigation into these regulators is warranted to determine if these proteins and/or associated pathways plays a role in the maintenance or modulation of DP cells to induce hair regeneration.

2020-11-16 | GSE149189 | GEO

Gene expression signatures for highly effective dermal sheath cup cells

Project description:In order to identify genes that correlate with efficacy in clinical trials, titled Autologous cell–based therapy for male and female pattern hair loss using dermal sheath cup cells, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish efficacy. RNA was extracted from each DSC-cells that was used in the clinical trial, and gene expression profiling by microarrays was performed. DSC cells derived from representative 8 IDs of highly effective and less effecgive group were analyzed. Signature was identified that distinguished between highly and less effective IDs.

2025-02-18 | GSE197025 | GEO

Human,scalp,dermal papilla cells,LC-MS/MS

Project description:Three individual patient-originated (Scalp trauma; ages 37, 46 and 57) DPCs were used for the studies. Dermal papilla cells, derived from the frontal scalp of 3 women, were treated with normal medium (Ctrl group) and medium containing 10-7 M corticotropin-releasing hormone (CRH group) for 72 h. Proteins were extracted and digested with trypsin for 4D label-free quantitative proteomics (Shanghai Applied Protein Technology Co., Ltd.).

2025-05-07 | PXD057745 | Pride

Single cell transcriptome of dermal sheath cup cells contributing to efficacy of cell-based therapy for patterned hair loss

Project description:Autologous cell-based therapy using dermal sheath cup (DSC) cell is a new approach for male and female pattern hair loss. While its efficacy was recently reported, the mechanism of action remains unclear. Furthermore, the differences in treatment response require clarification. First, we used multivariate analysis to categorize two groups of individuals: patients in which therapy showed high efficacy (HE group) and patients in which therapy showed low efficacy (LE group). Then We analyzed the differences in gene expression between DSC cells from the HE and LE groups by single-cel transcriptome.

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Gene expression profiling of the intact dermal sheath cup of human hair follicle

Project description:The dermal sheath cup is the peribulbar component of the hair follicle dermal sheath, and has hair inductive potential similar to the dermal papilla. To characterize it in comparison with other mesenchymal follicle tissuesparts, we performed gene expression profiling of intact dermal sheath cups, which were separated from hair follicles by microdissection. Gene expression profiles of the dermal sheath cup, dermal papilla and upper dermal sheath were compared. We identified a dermal sheath cup signature composed of 32 upregulated genes, which included extracellular matrix components and BMP binding mollecules, while dermal papilla signature included a number of dermal papilla signature genes which had already reported. Analyses of upstream regulators showed that TGF- b1 is a putative regulator of these genes. These results suggest some of molecular mechanism that contributes to human dermal sheath cup properties, which could be useful for hair follicle bioengineering.

2020-02-22 | GSE95219 | GEO

Dermal papilla signature gene expression in immortalized human dermal papilla cells from balding and non-balding scalp

Project description:Transcriptome analysis of DP signature gene expression in hTERT-immortalized balding (BAB) and non-balding (BAN) dermal papilla cells derived from frontal and occipital scalp of male patients with androgenetic alopecia Hamilton grade IV.

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Expression data obtained from primary dermal papilla cell cultures and human hair follicle organ culture from occipital scalp

Project description:In this dataset, we include the expression data obtained from primary dermal papilla cell cultures and human hair follicle organ culture from occipital scalp

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Global profiling of human hair follicle scalp dermal papilla cells using Affymetrix microarrays

Project description:Dermal papilla cells isolated from the human hair follicle are capable of inducing hair growth in recipient epithelia. However, demonstrating disparity from rodent dermal papilla, human cells lose this inductive competance immediately upon growth in culture under normal growth conditions. We grew dermal papilla cells in hanging drop cultures that are morphologically akin to intact dermal papilla, and found that by enhancing the environment for aggregation, we could restore the inductive capacity of human dermal papilla cells in culture. The underlying genes that regulate the inductive potential of dermal papilla cells is not well understood, and we sought to use global profiling to identify key genes and pathways related to inductive competance within dermal papilla cells. We used Affymetrix microarrays to profile human dermal papilla cells in both hair inducing, and non-hair inducing states. Affymetrix microarrays were used to to perform profiling of human dermal papilla cells, both as intact tissues (freshly isolated from scalp), and at several stages in subsequent two dimensional culture; cell explant outgrowths (p0), cells at passage 1 (p1), passage 3 (p3) and passage 5 (p5). RNA was isolated from cultured cells 72 hours after feeding. Cells at passage 3 were also grown in hanging drops to form dermal spheroids, that were used for RNA collection 48 hours after establishment. All experiments were performed using tissue from three biological replicates (#D5, D6, D7),

2013-10-20 | E-GEOD-44765 | biostudies-arrayexpress

Gene expression signatures for highly effective dermal sheath cup cells

Project description:Gene expression signatures for highly effective dermal sheath cup cells

| PRJNA808415 | ENA

Transcriptome analysis reveals differences in vasculature signalling between human dermal papilla cells from balding and non-balding scalp

Project description:Transcriptome analysis of hTERT-immortalized balding (BAB) and non-balding (BAN) dermal papilla cells derived from frontal and occipital scalp of male patients with androgenetic alopecia Hamilton grade IV. Interrogation of transcriptome differences between BAB and BAN after dihydrotestosterone (DHT, active metabolite of androgen) treatment revealed significant enrichment of vasculature-related genes among down-regulated genes in BAB compared to BAN.

2016-04-20 | GSE66664 | GEO

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