Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tanscriptional profile of primary endothelial cells (HUVEC) in ADA2 depleted setting and effect of Dipyridamole (DPM) treatment

ABSTRACT: Tanscriptional profile of primary endothelial cells (HUVEC) in ADA2 depleted setting and effect of Dipyridamole (DPM) treatment

PROVIDER: PRJNA632723 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11788500.fastq.gz	Fastqsanger.gz
	SRR11788501.fastq.gz	Fastqsanger.gz
	SRR11788502.fastq.gz	Fastqsanger.gz
	SRR11788503.fastq.gz	Fastqsanger.gz
	SRR11788504.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 144

Similar Datasets

Tanscriptional profile of primary endothelial cells (HUVEC) in ADA2 depleted setting and effect of Dipyridamole (DPM) treatment

Project description:Adenosine deaminase 2 deficiency (DADA2) is an inherited disorder which can cause vasculitis of medium-sized blood vessels. The disease is caused by mutations in the CECR1 gene, which encodes a key metabolic enzyme in the purine pathway. It is not clear how DADA2 leads to vasculitis, and there are currently limited treatment options. We, therefore, as a first step aimed to study the altered gene expression profile of primary endothelial cells in ADA2 depleted setting and then assessed the effect of Dipyridamole (DPM), a pharmacological inhibitor of ENT- transporters, on the differentially expressed genes. The whole genome transcriptome analysis revealed a robust induction of an IFNβ-stimulated gene signature in unstimulated siADA2-treated cells. Differentially expressed transcripts in this dataset included genes encoding pro-inflammatory cytokines, chemokines, and innate immune response proteins. Pre-treatment with dipyridamole (DPM), reduced expression of the IFNβ-driven gene signature upon depletion of ADA2.

2020-06-01 | GSE150540 | GEO

Differential expression of endogenous retroelements (long terminal repeats) in ADA2 depleted primary endothelial cells (HUVECs)

2020-06-20 | GSE151924 | GEO

The role of the central region of Ada2 in gene regulation

Project description:The SAGA complex of Saccharomyces cerevisiae contains greater than 20 components that acetylate and deubiquitylate nucleosomal histones. Its acetyltransferase, Gcn5 preferentially acetylates histones H3 and H2B and is regulated through interactions with Ada2 and Ngg1/Ada3. The N-terminal region of Ada2 contains a SANT domain that contacts Gcn5 near its catalytic site. Sequence alignments of Ada2 homologues indicate a conserved ~120 amino acid residue central region that interacts with Ngg1.To examine the function of this central region, we constructed ada2 alleles with mutations of clustered conserved residues. One of these alleles, ada2-RLR, resulted in a ~3-fold reduction in transcriptional activation of the PHO5 gene and growth changes that parallel deletion of ada2. Microarray analyses further revealed that ada2-RLR alters expression of a subset of those genes affected by deletion of ada2. Keywords: yeast, Ada2, SAGA complex, gene expression, genetic modification

2008-11-11 | GSE11377 | GEO

Impact of ADA2 on human macrophages

Project description:This study aims to understand the impact of adenosine deaminase 2 (ADA2) on human monocyte-derived macrophages

2021-09-24 | GSE184572 | GEO

Differential expression of endogenous retroelements (long terminal repeats) in ADA2 depleted primary endothelial cells (HUVECs)

Project description:Differential expression of endogenous retroelements (long terminal repeats) in ADA2 depleted primary endothelial cells (HUVECs)

| PRJNA637655 | ENA

RNA sequencing results of dipyridamole treatment in SLC29A2-expressing SK-hep1 cells

Project description:We observed an increasing trend of nuclear SLC29A2 expression associated with high histological de-differentiation grades, metastasis and poor patient prognosis by immunohistochemical analysis of HCC tumors. Treatments of dipyridamole to SLC29A2 expressing SK-hep1 cells reduced cell viability and cell migration. To examine the anticancer activities of dipyridamole, transcriptome analysis was performed to detect the modulations of oncogenic SLC29A2 and the downstream signaling pathways.

2020-10-23 | GSE139583 | GEO

Transcriptional network controlled by Candida glabrata Ada2, a transcription coactivator

Project description:In order to identify genes governed by Ada2, a transcription coactivator in C. glabrata, we compared the genome-wide expression profile in wild-type strain CBS138 and ada2 mutant via RNA sequencing. According to our analyses, 43 genes were down- and 443 genes were up-regulated in ada2 mutant when compared with the wild-type. The 43 down-regulated genes involved in ergosterol biosynthesis pathway (ERG6, ERG13), adhesin or adhesin-like genes (ICS2, AWP2, EPA7), heat shock genes (HSP30, SSB2) and other cellular functions. Among the 443 up-regulated genes, nine adhesin-like EPA family genes (EPA1, EPA6, EPA8, EPA10, EPA11, EPA12, EPA15, EPA20, EPA23) and three yapsin family genes (YPS4, YPS8, YPS10) were included.

2017-05-24 | GSE76338 | GEO

Dipyridamole, a compound that increases extracellular adenosine levels, as a preventive treatment for rheumatoid cachexia

Project description:Muscle decline due to chronic inflammation is a common condition in RA patients called rheumatoid cachexia (RC). Adenosine and ATP levels are essential in the control of myogenesis, anabolism/catabolism and senescence of muscle cells. Dipyridamole, an antiplatelet drug, is able to modulate adenosine levels in muscle cells and is an interesting therapeutic agent for RC. K/BxN mice model was treated with dipyridamole daily and sacrificed at the highest point of pawn inflammation. Cytokines arrays were performed in serum and muscle. Histology of TA and ankle was analyzed. Protein expression of myogenesis, purinergic system and senescence key markers were studied. Nucleotide content of GA was analyzed via HPLC. 2D and 3D culture models with C2C12 cells were performed to confirm the results obtained in mice model. RA mice developed weight loss, muscle size and activity decline and increment of senescence and atrophy. All this is dependent on the low adenosine levels and decreased expression of adenosine A2A/A2BR in RA muscle. Dipyridamole was able to prevent inflammation and muscle pathologies in RA mice due to adenosine and A2A/A2BR maintenance also cAMP/AMPK activation. This finding suggests dipyridamole is an interesting preventive drug to RA patients for counteracts RC development.

2026-07-08 | GSE274162 | GEO

Impact of ADA2 on human macrophages

Project description:Impact of ADA2 on human macrophages

| PRJNA765187 | ENA

GCN5-ADA2 Recruits ATP-Citrate Lyase (ACL) and Forms Condensates to Modulate Acetylation at H3K9 for Functional Genes in Rice Meristem

Project description:The SAGA complex is a highly conserved histone acetyltransferase (HAT) complex in eukaryotes, playing crucial role in regulating gene transcription throughout organismal development. The complex consists of two core components: GCN5, the HAT subunit, and ADA2, which primarily functions as an adaptor, facilitating interactions between GCN5 and other proteins and enhancing the complex’s accessibility to nucleosomes. Beyond its well-established roles, we discovered that, in rice, ADA2 possesses an evolutionary conserved intrinsically disordered region (IDR) that directs the SAGA complex to form nuclear condensates. Moreover, we identified a physical interaction between ADA2 and ATP-citrate lyase subunit A2 (ACLA2), leading to the formation of a GCN5-ADA2-ACLA2 (GAA) complex. Both GCN5 and ACLA2 exhibit phase separation in vivo, a process that is dependent on ADA2. Within these condensates, ACL contributes to the production and enrichment of acetyl-CoA, thereby promoting histone acetylation. Genetic evidence showed that knock-out or suppression of the three indicated genes collectively resulted in diminished meristem zones at both root tips and inflorescences, accompanied by a significant reduction in genomic H3K9 acetylation and transcriptional attenuation of essential genes for meristem function. In summary, our findings unveiled a novel mechanism for HAT in modulating gene transcription and plant development, in which the GAA complex phase separates and enriches acetyl-CoA metabolites within the nuclear puncta to facilitate the acetylation of histones at target genes.

2025-09-23 | GSE300491 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data