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RNA-array in DOT1L knockout and wild type CD8+ T cells

ABSTRACT: RNA-array in DOT1L knockout and wild type CD8+ T cells

PROVIDER: PRJNA633850 | ENA |

REPOSITORIES: ENA

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RNA-array in DOT1L knockout and wild type CD8+ T cells

Project description:Disruptor of telomeric silencing 1-like (DOT1L) is the specific and sole methyltransferase for H3K79. However, the role of Dot1l in T cells is not well determined. Here, used CD4-Cre/Dot1lflox/flox mice to specifically delete DOT1L in T cells. The genes characteristics were determined by RNA array and compared to wild type cells. We used microarrays to detail the global gene expression in Dot1l knockout and wild type CD8+ T cells.

2020-07-30 | GSE150845 | GEO

Transcription profiling by array of chicken wild type and tumor supressor Dot1L knockout lymphoblast cells

Project description:Effect of abrogation of the histone methyltransferase Dot1L on gene expression in the chicken DT40 model system

2011-05-07 | E-MEXP-2722 | biostudies-arrayexpress

Targeting the histone methyltransferase DOT1L rewires CD8 T cells and enhances their intrinsic cytotoxic activity towards tumor cells [RNAseq_WT_KO_DMSO_EPZ]

Project description:Insights into the epigenetic mechanisms of CD8 T cell differentiation are delivering novel opportunities for modulating fate decisions and immune responses. The histone methyltransferase DOT1L is emerging as a central epigenetic regulator in immune cells. However, its cell-intrinsic role in CD8 T cell programming remains unclear as positive as well as negative roles have been ascribed to DOT1L. Here, we determined the cell-intrinsic role of DOT1L in mouse CD8 T cells using conditional ablation. In contrast to deletion of Dot1L early in the T cell lineage, conditional ablation of Dot1L in isolated mature CD8 T cells in vitro did not compromise the in vivo proliferative capacity and anti-tumor reactivity. In vitro, Dot1L knock-out CD8 T cells showed accelerated and enhanced antigen-specific cytotoxic activity towards tumor cells. Mechanistically, transcriptome and proteome profiling revealed that loss of DOT1L results in an altered cell-identity program with loss of T-cell and gain of NK-cell features. This role of DOT1L was linked to its catalytic activity since treatment with specific DOT1L inhibitors phenocopied genetic deletion of Dot1L. Our findings show that ablation of DOT1L activity is well-tolerated in mature CD8 T cells, rewires their cell identity in a tunable way towards the NK-cell lineage and enhances their cytolytic activity cell intrinsically.

2025-11-03 | GSE282854 | GEO

Targeting the histone methyltransferase DOT1L rewires CD8 T cells and enhances their intrinsic cytotoxic activity towards tumor cells [RNAseq_DMSO_SGC]

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Targeting the histone methyltransferase DOT1L rewires CD8 T cells and enhances their intrinsic cytotoxic activity towards tumor cells [ChIPSeq_DMSO_EPZ_SGC]

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Gene expression analysis of P3 Dot1l conditional knockout mice in the cerebellum and of cerebellar granular neuron progenitors (CGNPs) or cerebellar granular neurons (CGNs) isolated from P7 wt mice upon DOT1L inhibition

Project description:DOT1L as methyltransferase of H3K79 is implicated in brian development. Here, we further defined DOT1L function in gene expression during cerebellar development using Microarrays. For that we generated Dot1l knockout mice using a Atoh-Cre driver line resulting in a Dot1l knockout within the cerebellum. The RNA of cerebellar tissue of the Dot1l knockout animals was thereby compared to controls. Additionally we compared the RNA levels of cultured CGNP and CGN samples treated with a DOT1L inhibitor versus DMSO treated cells. The data sets reveals potential new gene expression targets of DOT1L in vivo and in vitro, which ensure a correct development of the cerebellum.

2018-10-15 | GSE101945 | GEO

RNA expression in WT and Dot1L KO CD8 T cell subsets

Project description:We studied the role of the histone methyltransferase DOT1L in T cell development and differentiation. Here we generated RNA-seq data from sorted CD8 subpopulations from WT (Lcre+/-) and Dot1L KO (Lcre+/-;Dot1Lfl/fl) mice. This data was to define tissue specific expression signatures and it was compared with H3K79me2 ChIP-seq data from the same subsets.

2020-07-29 | GSE138910 | GEO

Transcription profiling of naive CD4+ and CD8+ T-cells from Gfi1-knockout and wild type mice

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2008-10-17 | E-GEOD-4938 | biostudies-arrayexpress

Expression profiling of WT and Dot1L KO CD8 effector T cells and H3K79me2 occupancy in WT effector CD8 T cells

Project description:We studied the role of the histone methyltransferase DOT1L in T cell development and differentiation. We generated RNA-seq data from sorted CD8 effector T cells from WT (Lck-cre+/-) and Dot1L KO (Lck-cre+/-;Dot1Lfl/fl) mice infected with Listeria monocytogenes. This data was compared with H3K79me2 ChIP-seq data from the same T cell subset.

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Next Generation Sequencing of CD8+ cells from Wild Type and miR-144/451 knockout mice

Project description:To investigate the function miR-144/451 on the function of CD8+ cells, we sorted the CD8a+ cells from wild type and miR-144/451 knockout mice, and then performed gene expression profiling analysis using data obtained from RNA-seq.

2022-05-11 | GSE202260 | GEO

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