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Wild-type and tafazzin-deficient clones of granulocyte-monocyte progenitors (GMP), ER-HOXB8 conditionally immortalized, immature, in estrogen.

ABSTRACT: Wild-type and tafazzin-deficient clones of granulocyte-monocyte progenitors (GMP), ER-HOXB8 conditionally immortalized, immature, in estrogen.

PROVIDER: PRJNA634362 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11827100.fastq.gz	Fastqsanger.gz
	SRR11827101.fastq.gz	Fastqsanger.gz
	SRR11827102.fastq.gz	Fastqsanger.gz
	SRR11827103.fastq.gz	Fastqsanger.gz
	SRR11827104.fastq.gz	Fastqsanger.gz

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Gene expression analysis of wild-type and Tafazzin-deficient granulocyte-monocyte progenitor (GMP) clones (ER-HOXB8 conditionally immortalized)

Project description:Gene expression analysis of wild-type and Tafazzin-deficient granulocyte-monocyte progenitor (GMP) clones (ER-HOXB8 conditionally immortalized)

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Wild-type and tafazzin-deficient clones of granulocyte-monocyte progenitors (GMP), ER-HOXB8 conditionally immortalized, immature, in estrogen.

Project description:Evaluate changes in gene expression resulting from the loss of the gene TAZ (protein tafazzin). Tafazzin is an enzyme involved in cardiolipin maturation and cardiolipin is a phospholipid found only in the inner mitochondrial membrane. Gene expression profiling was performed in a murine granulocyte-monocyte progenitor in vitro cell system. We identified a limited number of changes in gene expression, which was not surprising given that the tafazzin protein is localized in the mitochondria and has a very specific enzymatic activity. Genes involved in cholesterol and fatty acid metabolism (Acss1, Acat2, Ldlr), apoptosis (Bcl2, Casp3, Casp6, Cycs), and ER-stress induced apoptosis (Ddit3, Atf4) were differentially expressed.

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