Project description:ENO2, a bifunctional protein with enolase activity and transcriptional activity, plays a vital role in plant development and responses to environmental stresses. However, only several downstream genes targeted by ENO2 have been reported so far. Our study here identified hundreds of candidates of ENO2 targets with ChIP-seq and our special anti-ENO2 antibody. These results will help us further study the role of ENO2 in plant growth/development and environmental stress responses.
Project description:Drought is one of the most detrimental environmental factors that adversely affect crop production, thus jeopardizing food supplies for a growing world population. Over the past years, it has become evident that microorganisms associated with plants can enhance drought tolerance. However, the specific genetic and molecular mechanisms underpinning bacterial induction of drought tolerance in plants are still largely unknown.In our work, we have shown that a root endophytic Flavobacterium confers significant drought tolerance to Arabidopsis thaliana. We showed that the bacterium enhances drought tolerance by maintaining (and inducing) root hair formation and elongation under drought. By combining molecular, genetics, and omics technologies we identified an essential role of ethylene and two ethylene-induced transcription factors belonging to the ERF family (ERF115 and ERF114) through which flavobacterium exerts its effect on plant root hair formation and drought tolerance. Here we performed transcriptome analysis using RNA-seq to identify genes downstream of the two ERF transcription factors.