Project description:In the present study, we developed a chemical method to produce dihydro nicotinamide mononucleotide (NMNH), which is the reduced-form of nicotinamide mononucleotide (NMN). We demonstrated that NMNH was a better nicotinamide adenine dinucleotide (NAD+) enhancer compared to NMN both in vitro and in vivo mediated by mononucleotide adenylyltransferase (NMNAT). Additionally, NMNH increased the reduced NAD (NADH) levels in cells and in mouse liver. Metabolomic analysis revealed that NMNH inhibited glycolysis and TCA cycle. In vitro experiments demonstrated that NMNH induced cell cycle arrest and suppressed cell growth. Nevertheless, NMNH treatment did not cause observable difference in mice. Taken together, our work demonstrates that NMNH is a potent NAD+ enhancer, and suppresses glycometabolism and cell growth.
Project description:Dataset of HRMSMS profiles of 5 different specimen of Psiloxylon mauritianum. Collected on La Reunion island in the contexte of the FEDER PHAR project (GURDTI 2018-1829-0002370, EU-Region Reunion-French State national counter-part). Voucher specimen was submitted to the local herbarium (Conservatoire Botanique National de Mascarin, accession number REU025096). EtOAc extracts profiled using UHPLC-HRMSMS on a Q-Exactive Focus orbitrap MS. Data dependant. Pos ionization mode. Supplementary file contain the MZMine Batch file which processed the MzXML files.
Project description:House sparrow specimens were stained with four different iodine-based stains to increase contrast in microCT. Proteomic characterization of muscle and bone was performed on contralateral samples (before and after staining). Iodination was detected for all stains.
Analysis on samples from the Smithsonian National Museum of Natural History: USNM 657964, USNM 657968, USNM 657963, USNM 657967
Project description:This dataset represents woody plants recorded in 16 1-ha forest plots in an elevational gradient in Madidi National Park, Bolivia, ranging from lowland Amazonian moist forest and lowland dry forest to the treeline of the Andean Altiplano. This work was carried out by David Henderson and Jonathan Myers (Washington University in St. Louis), Sebastian Tello (Missouri Botanical Garden and University of Missouri, St. Louis), and Brian Sedio (University of Texas at Austin and Smithsonian Tropical Research Institute).
2022-10-18 | MSV000090549 | MassIVE
Project description:Barcoding the arthropod genera of NMNH