Project description:House sparrow specimens were stained with four different iodine-based stains to increase contrast in microCT. Proteomic characterization of muscle and bone was performed on contralateral samples (before and after staining). Iodination was detected for all stains.
Analysis on samples from the Smithsonian National Museum of Natural History: USNM 657964, USNM 657968, USNM 657963, USNM 657967
Project description:Direct analysis in real time (DART) surface analysis files and LC-MS proteomics files of a Tlingit ceremonial hat. DART was performed to evaluate what was present on the surface and also to help understand connection between two components of the hat. Proteomics was performed on small samples of material from around the hat to characterize the original adornment. The hat is housed at the Smithsonian Natural Museum of Natural History (E74339, E74441).
Proteomics data were searched with PEAKS 8.5 and Prosight Lite v1.4 Build 1.4.6
Project description:One tooth of a lamprey and one piece of trunc skin was lysed and analysed for its protein content. The samples were generously provided by the Museum of Natural History Vienna. The samples were stored in ethanol and the origin of the specimen is not known.
Project description:This study generated CRISPR barcode sequencing data from a PyMT-Cas9 mammary tumor cell line engineered with integrated homing guide RNA (hgRNA) lineage barcodes. Cells were collected at day 5 and day 14 following doxycycline-induced Cas9 activation to characterize barcode mutation accumulation and lineage evolution. These data were used to evaluate LINEMAP, a computational framework for quantitative reconstruction of lineage topology and cell division history from evolving CRISPR barcodes.
2026-06-15 | GSE335072 | GEO
Project description:Lichens at the US National Herbarium (NMNH, Smithsonian Institution)
Project description:Natural history museum specimens of historical honeybees have been successfully used to explore the genomic past of the honeybee, indicating fast and rapid changes between historical and modern specimens, possibly as a response to current challenges. In our study we explore a potential untapped archive from natural history collections - specimens of beeswax. We examine an Apis mellifera mellifera queen cell specimen from the 19th century. The intact and closed cell was analysed by X-ray Computed Tomography (CT) to reveal a perfectly preserved queen bee inside her cell. Subsequently, a micro-destructive approach was used to evaluate the possibility of protein extraction from the cell. Our results show that studies on specimens such as these provide valuable information about the past rearing of queens, their diet and development, which is relevant for understanding current honeybee behaviour. In addition we evaluate the feasibility of using historical beeswax as a biomolecular archive for ancient proteins to study honeybees.
