Project description:Here we have designed a full processing pipeline of P. falciparum RNA that innclues RNA preservation, extraction, cDNA synthesis and amplification, globin depletion and final transcriptome readout. To validate the protocol we have analyzed transcriptomes of P. falciaprum samples derived from filed studies as well as 3D7 lab strains. This dataset have been used to establish the best conditions for whole transcriptome amplification protocol. Additionally it inclues the validation of bead extraction protocol. It also contains field samples used for validation of the entire protocol.

2020-10-20 | GSE151754 | GEO

A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples.

Project description: Not available

| S-EPMC7547485 | biostudies-literature

Gene expression changes related to postnatal handling

Project description:Postnatal handling in rodents leads to decreased anxiety-like behavior in adulthood. We used microarrays to look at gene expression differences in the CA1 region of the hippocampus in female mice subjected to postnatal handling compared to controls.

2008-10-08 | GSE11679 | GEO

A guide to pre-processing high-throughput animal tracking data.

Project description: Not available

| S-EPMC9299236 | biostudies-literature

Time-dependent stability of plasma and liver interstitial fluid metabolites during sample handling in mice

Project description:<p>This study assesses the stability of metabolite levels in plasma and liver interstitial fluid (IF) from mice subjected to different handling times prior to extraction. Blood and tissues from female C57BL/6J mice were collected and either processed immediately or held on ice for 5, 10, or 30 minutes before analysis. Metabolite concentrations were quantified by LC/MS to determine how delays in processing influence metabolite integrity. The resulting data establish time-dependent changes in metabolite abundance during pre-analytical handling.</p>

2025-12-30 | MTBLS13164 | MetaboLights

Transcription profiling of rainbow trout liver in response to standardized handling stress

Project description:We constructed a targeted cDNA microarray consisting of 147 rainbow trout (Oncorhynchus mykiss) genes with known function to examine the transcriptional response to a standardized handling stress.

2006-01-31 | E-MEXP-622 | biostudies-arrayexpress

Handling of spurious sequences

Project description:Handling of spurious sequences dictates the outcome of high-throughput 16S rRNA gene amplicon profiling

| PRJEB34431 | ENA

The high-throughput human pri-miRNA processing assays

Project description:The high-throughput human pri-miRNA processing assays

| PRJNA890620 | ENA