Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single nucleus multi-omics regulatory atlas of the murine pituitary [single nuclei ATAC-Seq]

ABSTRACT: Single nucleus multi-omics regulatory atlas of the murine pituitary [single nuclei ATAC-Seq]

PROVIDER: PRJNA637896 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11945367_1.fastq.gz	Fastqsanger.gz
	SRR11945367_2.fastq.gz	Fastqsanger.gz
	SRR11945368_1.fastq.gz	Fastqsanger.gz
	SRR11945368_2.fastq.gz	Fastqsanger.gz
	SRR11945369_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Single nucleus multi-omics regulatory atlas of the murine pituitary [single nuclei ATAC-Seq]

Project description:The pituitary regulates growth, reproduction and other endocrine systems. To investigate transcriptional network epigenetic mechanisms in this gland, we generated paired single nucleus (sn) transcriptome and chromatin accessibility profiles in single mouse pituitaries, and genome-wide sn methylation datasets. Our analysis provided insight into cell type epigenetics, regulatory circuit and gene control mechanisms. Latent variable transcriptome and accessibility data representation resolved both inter-sexual and inter-individual variation in gene control programs. Multi-omics analysis of cell type-specific gene regulatory networks distinguished distinct mechanisms. Particularly, the FoxL2 gonadotrope network is controlled both by FoxL2 expression and by target gene epigenetic status. Co-accessibility analysis comprehensively identified putative regulatory regions, including a region that overlapped the fertility-linked rs11031006 human polymorphism. In vitro CRISPR-deletion at this locus increased Fshb levels, supporting this domain’s predicted regulatory role. The public pituitary atlas [link] is a resource for elucidating cell-type specific gene regulatory mechanisms and principles of transcription circuit control.

2021-02-23 | GSE151960 | GEO

Single nucleus multi-omics regulatory atlas of the murine pituitary

Project description:Single nucleus multi-omics regulatory atlas of the murine pituitary

| PRJNA637893 | ENA

Single nucleus multi-omics regulatory atlas of the murine pituitary [snRNA-seq ]

2021-02-23 | GSE151961 | GEO

Single nucleus multi-omics regulatory atlas of the murine pituitary [scRNA-Seq]

2021-02-23 | GSE151958 | GEO

Single nucleus multi-omics regulatory atlas of the murine pituitary [scRNA-Seq]

Project description:Single nucleus multi-omics regulatory atlas of the murine pituitary [scRNA-Seq]

| PRJNA637894 | ENA

Single nucleus multi-omics regulatory atlas of the murine pituitary [snRNA-seq ]

Project description:Single nucleus multi-omics regulatory atlas of the murine pituitary [snRNA-seq ]

| PRJNA637895 | ENA

A single-cell transcriptomic atlas of mouse pituitary aging

Project description:Aging is a multifactorial process with significant functional alterations of the human body including endocrinal systems which control the whole-body physiology and metabolism. In this vein, aging-induced decline of endocrine function are associated with multiple physiological and metabolic diseases. However, aging-associated molecular shifts in the pituitary gland, the central organ of the endocrine system, have not been dissected systemically. In this study, we conducted single-cell transcriptomic analysis of the anterior pituitary gland by comparing old and young male mice. Single-cell transcriptomics not only increased the resolution for clustering of various cell types in the pituitary gland, but also enabled detailed analysis of differential expression and intercellular communication caused by aging. In summary, our study constructed the first single-cell transcriptomic atlas of pituitary aging and identified associated features of in a single-cell level, providing resources to develop novel potential therapeutic targets for aging-associated endocrine dysfunction.

2023-09-08 | GSE242296 | GEO

A single-cell transcriptomic atlas of mouse pituitary aging

Project description:A single-cell transcriptomic atlas of mouse pituitary aging

| PRJNA1013052 | ENA

Single Nucleus Methylation Sequencing of Mouse Pituitary

Project description:The pituitary regulates growth, reproduction and other endocrine systems. To investigate transcriptional network epigenetic mechanisms, we generated paired single nucleus (sn) transcriptome and chromatin accessibility profiles in single mouse pituitaries, and genome-wide sn methylation datasets. Our analysis provided insight into cell type epigenetics, regulatory circuit and gene control mechanisms. Latent variable pathway analysis detected corresponding transcriptome and chromatin accessibility programs showing both inter-sexual and inter-individual variation. Multi-omics analysis of gene regulatory networks identified regulons whose composition and function within specific cell types were shaped by the promoter accessibility state of target genes. Co-accessibility analysis comprehensively identified putative cis-regulatory regions, including a domain upstream of Fshb that overlapped the fertility-linked rs11031006 human polymorphism; In vitro CRISPR-deletion at this locus increased Fshb levels, supporting this domain’s inferred regulatory role. The sn pituitary multi-omics atlas [link] is a public resource for elucidating cell-type specific gene regulatory mechanisms and principles of transcription circuit control. This GEO series contain raw (FASTQ) and processed data (ALLC) files for the sn methylation dataset generated by the snmC-seq2 method.

2021-02-23 | GSE152011 | GEO

Transcriptomic atlas of thalamic nuclei

Project description:Transcriptomic atlas of thalamic nuclei

| PRJNA552996 | ENA