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Targeted genomic sequencing of the TCRγ locus in ILC2s and γδT cells

ABSTRACT: Targeted genomic sequencing of the TCRγ locus in ILC2s and γδT cells

PROVIDER: PRJNA640276 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12042905_1.fastq.gz	Fastqsanger.gz
	SRR12042905_2.fastq.gz	Fastqsanger.gz
	SRR12042906_1.fastq.gz	Fastqsanger.gz
	SRR12042906_2.fastq.gz	Fastqsanger.gz
	SRR12042907_1.fastq.gz	Fastqsanger.gz

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Targeted genomic sequencing of the TCRγ locus in ILC2s and γδT cells

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Project description:The role of gamma-delta T (γδT) cells in immune responses to common allergens is poorly understood. Here, we utilized single-cell (sc) transcriptomic analysis of allergen-reactive γδT cells in humans to characterize the transcriptional landscapes and TCR repertoires in response to cockroach (CR) and mouse (MO) allergens. Using a novel Activation-Induced Marker (AIM) assay that allows detection of γδT cells combined with scRNA sequencing and TCR repertoire analysis, we identified both shared and allergen-specific γδT cell activation patterns and gene expression profiles. While CR extract activated both Vδ1 and Vδ2 subsets, MO extract primarily stimulated Vδ2 cells. Our analysis revealed allergen-specific clusters with distinct functional signatures, including enhanced inflammatory responses and cytotoxic effector functions in MO-specific γδT cells and natural killer cell-mediated immunity and IFNγ signaling in CR-specific populations. Comparison of allergic and non-allergic individuals highlighted differences in gene expression and TCR repertoires, including a higher IFNG expression in the CR-allergic compared to non-allergic cohorts, suggesting that phenotypic and functional differences are associated with γδT allergen responses. This study provides insights into the cellular and molecular heterogeneity and functionality of allergen-reactive γδT cells, offering a foundation for understanding their role in allergic diseases and potential therapeutic interventions.

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