Project description:We described an actinobacterial strain Tsukamurella tyrosinosolvens PS2 capable of active utilization of linear alkanes, producing bio-surfactants, and containing two alkane hydroxylation systems. Proteomics approaches have shown the significance of differentially expressed proteins responding to hydrocarbon adverse pressure, including increased expression of the stress and resistance proteins.
Project description:In this work, a characterization of the secretome of the alkane utilizing bacteria Tsukamurella tyrosinosolvens PS2 was carried out. capable of utilizing alkanes as the sole source of carbon and energy was carried out. The cell-free supernatant of the strain PS2 was analyzed during growth on hexadecane or sucrose. The analysis was conducted using timsTOF instrument.