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ABSTRACT: Negative plant-inoculum feedback limits productivity in aquaponics

PROVIDER: PRJNA641448 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12080442_1.fastq.gz	Fastqsanger.gz
	SRR12080443_1.fastq.gz	Fastqsanger.gz
	SRR12080444_1.fastq.gz	Fastqsanger.gz
	SRR12080445_1.fastq.gz	Fastqsanger.gz
	SRR12080446_1.fastq.gz	Fastqsanger.gz

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Oleic Acid Promotes Lipid Accumulation in Bovine Adipocytes: The Role of PPARα Activity

Project description:Background: Excessive lipid mobilization due to negative energy balance (NEB) in periparturient dairy cows increases their risk for diseases and decreases their productivity. Improving energy intake during the immediate postpartum limits NEB. One way to enhance energy density of the diet is through fatty acid (FA) supplementation. In periparturient cows, oleic acid (OA) limits lipolysis and improves adipose tissue (AT) insulin sensitivity. However, the mechanisms behind OA effect are still unknown. Our objective was to characterize the effect of OA, alone and in combination with palmitic acid (PA), on lipid accumulation, and identify a mechanism of action in bovine adipocytes. Results: Compared with CON, FA treatments had no effect on preadipocyte differentiation (P = 0.65). The treatments 300PA, 200OA, 300OA, 60-40, 50-50, and 40-60 increased lipid accumulation compared with CON (P < 0.0001). At d 7, 300PA and 300OA increased PPARα protein expression compared with CON (P = 0.04). PPARα inhibition decreased triglyceride levels of OA and 60-40 (P<0.05) but not for PA (P=0.26).

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Expression profile of Medicago truncatula roots inoculated with human enteric pathogens.

Project description:Disease outbreaks due to the consumption of legume seedlings contaminated with human enteric bacterial pathogens like Escherichia coli O157:H7 and Salmonella enterica are reported every year. We found surface and internal colonization of Medicago truncatula by Salmonella enterica and Escherichia coli O157:H7 even with inoculum levels as low as two bacteria per plant. Expression analyses using microarray revealed that some Medicago truncatula genes were regulated in a similar manner in response to both of these enteric pathogens. Medicago truncatula roots were inoculated with low inoculum levels of two enteric bacteria per plant (E. coli O157:H7 and Salmonella). 10 days post inoculated plants were used for RNA extraction and hybridization on Affymetrix microarrays.

2013-12-18 | E-GEOD-53406 | biostudies-arrayexpress

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