Dataset Information

Spore inoculum size impacts substrate degradation and sporulation but not the secretome during colonization of whole yellow pea (Pisum sativum) by Aspergillus oryzae

ABSTRACT: Aspergillus oryzae is used in solid-state fermentation (SSF) to produce plant-based foods. To this end, the substrate is inoculated with spores of this fungus. So far, the effect of inoculum size on SSF with A. oryzae has primarily focused on the production of specific enzymes. Therefore, the aim of this study was to examine the impact of inoculum size on the full secretome, combined with enzyme activity assays, assessment of colonization, substrate degradation, and sporulation. To this end, A. oryzae was grown for 7 days on whole yellow pea (Pisum sativum). Fluorescence microscopy with a GFP-expressing A. oryzae strain showed that peas had been colonized externally and internally, irrespective of inoculum size. Yet, the highest inoculum size resulted in a stronger pea biomass reduction when compared to the lowest inoculum size. By contrast, sporulation decreased with increasing inoculum size. Notably, proteomics revealed no effect of inoculum size on the protein profiles of aqueous extracts of the colonized peas. Amylases and proteases were the most abundant secreted proteins, which was consistent with their high activity in the aqueous extracts. Proteomics also identified β-1,3-glucanases and chitinases, indicating hyphal lysis. Indeed, 10–19% of the fungal proteins detected in the aqueous extracts lacked signal peptides. These data contribute to our understanding of colonization of substrates by A. oryzae and may be used to optimize SSF with this food grade fungus.

INSTRUMENT(S):

ORGANISM(S): Pisum Sativum (garden Pea) Aspergillus Oryzae Rib40

TISSUE(S): Whole Body, Fungal Cell

SUBMITTER: Nadzeya Staliarova

LAB HEAD: Kelly E. Stecker

PROVIDER: PXD070642 | Pride | 2026-06-26

REPOSITORIES: Pride

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			Action	DRS
	20231129_EX3_UM1_7838799_SA_EXT00_r1_open_E4_2ul.mgf	Mgf
	20231129_EX3_UM1_7838799_SA_EXT00_r1_open_E4_2ul.mzid	Mzid
	20231129_EX3_UM1_7838799_SA_EXT00_r1_open_E4_2ul.raw	Raw
	20231129_EX3_UM1_7838799_SA_EXT02_r1_open_E7_2ul_85min_gr.mgf	Mgf
	20231129_EX3_UM1_7838799_SA_EXT02_r1_open_E7_2ul_85min_gr.mzid	Mzid

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Spore inoculum size impacts substrate degradation and sporulation but not the secretome during colonization of whole yellow pea (Pisum sativum) by Aspergillus oryzae.

Braat Maaike M Braat Nienke N Staliarova Nadzeya N Vellozo-Echevarría Tomás T Siwek Hania M HM Kemmer Julius F JF Barrett Kristian K Meyer Anne S AS Stecker Kelly E KE Wösten Han A B HAB

International journal of food microbiology 20260519

Aspergillus oryzae is used in solid-state fermentation (SSF) to produce plant-based foods. To this end, the substrate is inoculated with spores of this fungus. So far, the effect of inoculum size on SSF with A. oryzae has primarily focused on the production of specific enzymes. Therefore, the aim of this study was to examine the impact of inoculum size on the full secretome, combined with enzyme activity assays, assessment of colonization, substrate degradation, and sporulation. To this end, A. ...[more]

PMID: 42176576

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