Project description:To understand the mechanism of action of ILB we have used gene expression analysis to document the changes induced by the drug in Schwann like cells. Human Schwann-like cells (ATCC-CRL-2884) were treated with ILB (0.01 mg/ml) for 48 hours. Control samples were cultured parallel with no drug added. The expression data was used to identify the ILB-regulated genes.
Project description:Schwann cells undergo reprogramming after nerve injury, switching to immature repair phenotype. The goal was to test what genetic perturbations are triggered in Schwann cells with the stimulus from melanoma cell secreted factors. We used microarrays to detail the changes in gene expression of Schwann cells treated with human melanoma conditioned medium.
Project description:We used microarrays to detail the transcriptome-wide gene expression changes underlying chemical conversion of human fibroblasts into induced Schwann Cells over a time period of 39 days. We compared then the expression profiles of these induced Schwann Cells to primary Schwann cells. The gene expression results analyzed in this study are further described in Thoma et al. (2014) Chemical conversion of human fibroblasts into functional Schwann cells. Under submission
Project description:In this study we compare transcriptional changes induced by mechanical compression in Schwann cells and pancreatic cancer cells. Human HEI-286 Schwann cells and pancreatic cancer cells MiaPaCa-2 and Panc01 were compressed at 5 μm height for 5 minutes in 6-wells static confiner (4Dcell) or uncompressed (not confined or confined in a 20 μm height space). Our data provides comparison of molecular response of Schwann cells and cancer cells to mechanical forces.
Project description:In this study we present transcriptional changes in HEI-286 Schwann cells upon compression. Human HEI-286 Schwann cells were uncompressed, compressed at 5 μm height for 5 minutes or for 4 hours in 6-wells static confiner to monitor continuous molecular response to compression.