Expression data from imbibed wheat seeds developed at different temperatures
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ABSTRACT:
PROVIDER: PRJNA642897 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptomic analysis of maintenance of dormancy induced by seed development temperature We used Affymetrix GeneChip Wheat Genome Array to detail transcriptional programs underlying maintenance of dormancy induced by seed development tempearture in imbibing seeds of wheat
2021-06-30 | GSE153527 | GEO
Project description:Wheat seed germination and seminal root growth can be inhibited by treatment with exogenous ABA We used Affymetrix GeneChip Wheat Genome Array to detail transcriptional programs affected by ABA during imbibition After-ripened seeds imbibed in ABA for 24 h were used for RNA extraction and hybridization on Affymetrix GeneChip. After-ripened seeds were generated by storing dormant seeds at room temperature for 10 months.
2013-06-05 | E-GEOD-32409 | biostudies-arrayexpress
Project description:Wheat seed dormancy is released by after-ripening, and germination and seminal root growth of after-ripened/non-dormant seeds can be inhibited by treatment with exogenous ABA. We used Affymetrix GeneChip Wheat Genome Array to detail transcriptional programs affected by after-ripening of dormant seeds and imbibition of after-ripened seeds in ABA.
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Project description: Not available
| S-EPMC10507141 | biostudies-literature
Project description:Transcriptional profiling of wheat embryos of developing seed comparing seeds grown at low temperature:13˚C with seeds grown at high temperature:25˚C during seed development using wheat 2 cultivars: Norin61 (N61) and Shiroganekomugi (SK). Goal was to determine the effects of temperature on global gene expression. Two-condition experiment, Low(13˚C) vs. high (25˚C) temperatures during seed development. Time course experiments along with days after anthesis (DAA).
2011-09-07 | E-GEOD-22786 | biostudies-arrayexpress
Project description:Keeping imbibed seeds at low temperatures for a certain period, so called seed vernalization (SV) treatment, promotes seed germination and subsequent flowering in various plants. Vernalization-promoting flowering requires GSH. However, the expression patterns analyzed by GeneChip arrays showed that increased GSH biosynthesis partially mimics SV treatment in Arabidopsis thaliana. SV treatment (keeping imbibed seeds at 4°C for 24 h) induced a specific pattern of gene expression and promoted subsequent flowering in wild-type plants. A similar pattern was observed at 22°C in transgenic plants (35S-GSH1 plants) overexpressing the γ-glutamylcysteine synthetase gene GSH1, coding an enzyme limiting GSH biosynthesis, under the control of the cauliflower mosaic virus 35S promoter. This pattern was strengthened at 4°C but flowering was less responsive to SV treatment. There was a difference in the transcript behaviour of the flowering repressor FLC between wild-type and 35S-GSH1 plants. Unlike other genes responsive to SV treatment, SV-dependent decrease in FLC in wild-type plants was reversed in 35S-GSH1 plants. SV treatment increased GSSG level in wild-type seeds, whereas GSSG level was high in 35S-GSH1 plants, even at a non-vernalizing temperature. Taking into consideration that low temperatures stimulate GSH biosynthesis and bring about oxidative stress, GSSG is considered to trigger low temperature response, but enhanced GSH synthesis was not enough for mimicking SV treatment. To complete it, it essentially required the cellular redox retransition from the oxidized to the reduced state that is observed after the seed vernalization treatment.
2012-06-01 | GSE33978 | GEO
Project description:Keeping imbibed seeds at low temperatures for a certain period, so called seed vernalization (SV) treatment, promotes seed germination and subsequent flowering in various plants. Vernalization-promoting flowering requires GSH. However, the expression patterns analyzed by GeneChip arrays showed that increased GSH biosynthesis partially mimics SV treatment in Arabidopsis thaliana. SV treatment (keeping imbibed seeds at 4°C for 24 h) induced a specific pattern of gene expression and promoted subsequent flowering in wild-type plants. A similar pattern was observed at 22°C in transgenic plants (35S-GSH1 plants) overexpressing the γ-glutamylcysteine synthetase gene GSH1, coding an enzyme limiting GSH biosynthesis, under the control of the cauliflower mosaic virus 35S promoter. This pattern was strengthened at 4°C but flowering was less responsive to SV treatment. There was a difference in the transcript behaviour of the flowering repressor FLC between wild-type and 35S-GSH1 plants. Unlike other genes responsive to SV treatment, SV-dependent decrease in FLC in wild-type plants was reversed in 35S-GSH1 plants. SV treatment increased GSSG level in wild-type seeds, whereas GSSG level was high in 35S-GSH1 plants, even at a non-vernalizing temperature. Taking into consideration that low temperatures stimulate GSH biosynthesis and bring about oxidative stress, GSSG is considered to trigger low temperature response, but enhanced GSH synthesis was not enough for mimicking SV treatment. To complete it, it essentially required the cellular redox retransition from the oxidized to the reduced state that is observed after the seed vernalization treatment. Four samples (Col-0 and 35S-GSH1 seeds imbibed at 22˚C or 4˚C). Two replicates for each samples.
2012-05-31 | E-GEOD-33978 | biostudies-arrayexpress
Project description:Transcriptional profiling of wheat embryos of developing seed comparing seeds grown at low temperature:13˚C with seeds grown at high temperature:25˚C during seed development using wheat 2 cultivars: Norin61 (N61) and Shiroganekomugi (SK). Goal was to determine the effects of temperature on global gene expression.
2011-09-08 | GSE22786 | GEO
Project description:We analyzed the transcriptome of dormant and after-ripened imbibed seeds of the Arabidopsis accession Cape verde Islands. We analyzed the transcriptional changes in temporal and spatial detail by sampling at four imbibition time-points (3, 7, 12 and 24 hours after sowing) and two seed compartments (RAD and MCE).
2016-08-24 | GSE76907 | GEO
Project description:We analyzed the transcriptome of dormant and after-ripened imbibed seeds of the Arabidopsis accession Cape verde Islands. We analyzed the transcriptional changes in temporal and spatial detail by sampling at four imbibition time-points (3, 7, 12 and 24 hours after sowing) and two seed compartments (RAD and MCE). Gene expression in imbibed dormant and after-ripened seeds was compared. For each sample three replicates were used.
2016-08-24 | E-GEOD-76907 | biostudies-arrayexpress