Project description:Raw metaproteomic data of Chinese indoor dust derived extracellular vesicles. These dust samples were collected in the summer of 2022 and were immediately sent to the laboratory for processing. The extracellular vesicles of dust were subjected to ultracentrifugation, and they were further purified using iodixanol density gradient ultracentrifugation. After extracting the extracellular vesicles, metaproteomic sequencing was performed.
Project description:Occupational exposure to dust containing crystalline silica may result in serious adverse health effects including silicosis and cancer. Previous studies which employed animal models for inhalation exposure to crystalline silica revealed changes in blood gene expression profiles in association with the silica-induced lung toxicity. Currently, global gene expression profiles were determined in the whole blood samples obtained from control (not exposed to dust) and dust containing silica exposed individuals with or without clinically identified silicosis. Differences in the blood gene expression profiles were detected in the blood samples obtained from the control and the silica-containing dust exposed individuals. Between the two groups of the silica-containing dust exposed individuals, the number of significantly differentially expressed genes was more in the blood samples obtained from those with silicosis.
Project description:This study underscores the urgent need to understand how lunar regolith dust affects human health, an issue critical for sustaining long-term human presence on the Moon. Past Apollo missions, revealed that lunar dust exposure can trigger allergy-like symptoms, suggesting a biological reactivity that is still poorly understood. Using a 3D human airway model that closely mimics real bronchial tissue, researchers found that lunar regolith simulant (JSC-1) causes unique and early physiological changes that differ significantly from responses to Earth-based dust (PM10). The distinct effects of lunar regolith on mucociliary clearance, barrier integrity, and airway remodeling suggest that cellular and molecular pathways are being activated or suppressed in ways not seen with terrestrial particles. Proteomics can uncover which specific proteins are altered during exposure, providing insight into the mechanisms of toxicity, stress response, and long-term adaptation or damage. Moreover, the data reinforces that terrestrial dust studies are not directly translatable to the lunar context. Therefore, proteomic profiling specific to lunar dust exposure is not just beneficial, it's necessary to develop targeted medical countermeasures, design safer habitats, and support the health of future lunar explorers.
Project description:Investigation of the gene expression changes associated with TLR2 adjuvant (lipoteichoic acid; LTA) inhibition of allergic TH2 responses in peripheral blood mononuclear cell cultures (derived from house dust mite allergic individuals) stimulated with house dust mite extract. Pooled samples from 5 individuals were analysed by microarray; qPCR validation was carried out in a larger cohort of 23 individuals.
Project description:To identify relevant mRNA targets of differentially dysregulated lung miRNA signatures of a house dust mite mouse (HDM) model of asthma. cDNA libraries were constructed using the same lung RNA samples as the miRNA libraries preparation.
Project description:This study are compares the transcriptomes of lower airway constitutive connective tissue mast cells (MCs), characterized by low expression of b7 integrin (B7low), and induced mucosal MCs, characterized by high b7 integrin (B7hi) at rest and in response to repeated inhalation of house dust mite extract (HDM). Lower airway b7hi and b7low MCs were flow cytometrically isolated from female 5-7 week old C57BL/6 mice following 6 challenges (2x/week for three weeks) with saline (PBS) or 3 ug house dust mite extract (HDM). Each sample is derived from pooled lung digests from three mice to reduce experimental variance. Samples were sequenced on an Illumina NextSeq 500