Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profile of tumor associated macrophages in High fat diet mouse models

ABSTRACT: Transcriptional profile of tumor associated macrophages in High fat diet mouse models

PROVIDER: PRJNA644630 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12168644_1.fastq.gz	Fastqsanger.gz
	SRR12168644_2.fastq.gz	Fastqsanger.gz
	SRR12168645_1.fastq.gz	Fastqsanger.gz
	SRR12168645_2.fastq.gz	Fastqsanger.gz
	SRR12168646_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

Transcriptional profile of tumor associated macrophages in High fat diet mouse models

Project description:Tumor associated macrophages (TAMs) are correlated with the progression of prostatic adenocarcinoma (PCa) but are still poorly described in this context. Here we applied high dimensional single-cell RNA-seq to profile the transcriptional landscape of TAMs in PCa. We identified a subset of tumor infiltrating macrophages that shows a dysregulation in transcriptional pathways associated with lipid metabolism. In human and mouse models of PCa this subset of macrophages expresses the scavenger receptor MARCO and is characterized by accumulation of lipid droplets. We identified a gene signature derived from MARCO-expressing TAMs that correlates with PCa progression and is associated with shorter disease-free survival. Mechanistically, we observed that lipid accumulation in TAMs is promoted by the secretome of cancer cells. Lipid-loading confers on tumor-conditioned macrophages the capability to promote cancer cell migration mediated by CCL6. Importantly, administration of high fat diet to tumor-bearing mice raises lipid accumulation in TAMs and affects their transcriptional profile. Finally, we demonstrate that the accumulation of lipids in macrophages is dependent on MARCO expression and we show that MARCO blockade hinders tumor growth and invasiveness in models of advanced PCa. Our findings provide evidence that lipid-loaded TAMs represent a new therapeutic target in PCa.

2022-02-16 | GSE153977 | GEO

Transcriptomic profile of replicating NAFLD hepatocytes (from High-Fat High-Sucrose Diet - HFHS and Choline-Deficient High-Fat Diet - CDHFD).

Project description:C57Bl6/J male mice were put on different diets at 5 weeks of age, with a standard diet (SD) or a High-Fat High-Sucrose Diet (HFHS) or a Choline-Deficient High-Fat Diet (CDHFD) during 6 months. Primary hepatocytes cultures from 3 different models were synchronized in the cell cycle. Transcriptomic analysis was perfomed at 48hours of culture when HFHS and CDHFD hepatocytes harbor replication stress.

2020-07-11 | GSE154194 | GEO

High-Fat-Diet

Project description:High Fat Diet Experiment SD rats fed either an AIN93G-based low fat diet or an AIN93G-based high fat diet. Keywords: ordered

2006-01-01 | GSE2226 | GEO

Gene expression profile at single cell level of mouse heart from control diet (CD), high fat diet (HFD) and Western diet (WD)

Project description:Macrophages are considered as a homogeneous population in the heart. We used single cell RNA sequencing (scRNA-seq) to analyze thediversity of macrophages in control diet (CD) mouse, high fat diet (HFD) mouse and Western diet (WD) mouse heart.

2024-08-19 | GSE266363 | GEO

Transcriptional profiling of hepatic macrophages isolated from mice with high-fat diet -induced non-alcoholic fatty liver disease

Project description:Purpose: The goals of this study were to identify preferential gene expression signatures that are unique to hepatic macrophages in high-fat diet -induced non-alcoholic fatty liver disease. Methods and results: Wild-type and Casp11-/- mice were treated with high fat and normal chow diet for a period of 12 weeks. Hepatic macrophages from liver were isolated to generate mRNA transcription. Conclusion: Our study represents detailed analysis of caspase-11 in regulating hepatic macrophages in high-fat diet -induced non-alcoholic fatty liver disease.

2023-01-17 | GSE221005 | GEO

Single-cell RNA-sequencing of mouse colonic monocytes and macrophages (chow vs. high fat diet)

Project description:We performed a single-cell transcriptome analysis of colon monocytes and macrophages in 2 mice fed with control diet (chow) and 2 mice fed with high-fat diet for 1 week.

2022-04-27 | GSE143351 | GEO

Transcriptomic profiling of cardiac resident macrophages upon high-fat diet or with aging

Project description:Cardiac resident macrophages (CRM) are essential for cardiac homeostais and physiological/pathological responses. CRM of high-fat diet fed mice or aged mice were sorted by FACS and subjected to SMART-seq.

2024-01-24 | GSE253651 | GEO

Effect of high fat diet feeding on adipose tissue macrophages

Project description:Effect of high fat diet feeding on adipose tissue macrophages

| PRJNA706728 | ENA

Mouse muscle mitochondrial proteome changes in high fat diet vs. low fat diet

Project description:We investigated remodeling of the mitochondrial proteome to determine mechanisms of changes to lipid oxidation following high-fat feeding. C57BL/6J mice consumed either a high-fat diet (HFD, 60% fat) or low fat diet (LFD, 10% fat) for 12 weeks. Mice were fasted 4 hours then anaesthetized by sodium pentobarbital for tissue collection. A mitochondrial-enriched fraction was prepared from gastrocnemius muscles and underwent proteomic analysis by high-resolution mass spectrometry.

2018-04-12 | MSV000082272 | MassIVE

Murine Mammary fat pad on high fat diet

Project description:Younger age and obesity increase the incidence and rates of metastasis of triple-negative breast cancer (TNBC), an aggressive subtype of breast cancer. The tissue microenvironment, specifically the extracellular matrix (ECM), is known to promote tumor invasion and metastasis. We sought to characterize the effect of both age and obesity on the ECM of mammary fat pads. We used a diet-induced obesity (DIO) model where 10-week-old female mice were fed a high-fat diet (HFD) for 16 weeks or a control chow diet (CD) where time points were every 4 weeks to monitor age and obesity HFD progression. We isolated the mammary fat pads to characterize the ECM at each time point. Utilizing proteomics, we found that the early stages of obesity were sufficient to induce distinct differences in the ECM composition of mammary fat pads that promote TNBC cell invasion. ECM proteins previously implicated in driving TNBC invasion Collagen IV and Collagen VI, were enriched with weight gain. Together these data implicate ECM changes in the primary tumor microenvironment as mechanisms by which age and obesity contribute to breast cancer progression.

2024-06-06 | PXD050250 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data