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Expression profiling in cohesin mutant MCF10A epithelial and CMK leukaemia cells

ABSTRACT: Expression profiling in cohesin mutant MCF10A epithelial and CMK leukaemia cells

PROVIDER: PRJNA645097 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12183775_1.fastq.gz	Fastqsanger.gz
	SRR12183775_2.fastq.gz	Fastqsanger.gz
	SRR12183776_1.fastq.gz	Fastqsanger.gz
	SRR12183776_2.fastq.gz	Fastqsanger.gz
	SRR12183777_1.fastq.gz	Fastqsanger.gz

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Expression profiling in cohesin mutant MCF10A epithelial and CMK leukaemia cells

Project description:MCF10A cells were CRISPR-Cas9 edited to create heterozygous deletion in RAD21 and SMC3 subunits of cohesin. STAG2 is on the X chromosome, hence CRISPR-Cas9 editing resulted in complete loss of STAG2. Total RNA was sequenced from the MCF10A parental and cohesin mutant MCF10A lines. The acute megakaryoblastic leukaemia cell line CMK was CRISPR-Cas9 edited to cotain STAG2 R614* mutation. CRISPR-Cas9 edited STAG2 mutant line showed complete loss of STAG2. CMK parental and the STAG2 mutant line were treated with Wnt3a for 4 hours and total RNA was sequenced at in the control or non-treated (con) and following 4 hours of Wnt3a treatment (Wnt3a4hr).

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