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Antagonist actions of CMK-1/CaMKI and TAX-6/Calcineurin along the C. elegans thermal avoidance circuit orchestrate nociceptive habituation

ABSTRACT: In this project, we performed three sets of in vitro kinase assays coupled to phosphoproteomic workflows. (1) tryptic peptides of C. elegans lysate treated with recombinant active CMK-1 (positive2 run) and kinase-dead CMK-1 and 18O labeled ATP (2) immobilized whole cell lysate (native conditions, protein level assay) incubated without ATP (negative control 1), with ATP (negative control 2), and with ATP and with active CMK-1 (positive experiment). After tryptic digestion, peptides were dimethyl-labeled using standard conditions. (3) purified TAX-6 isoform A and C were treated with recombinant active CMK-1 and kinase-dead CMK-1.

INSTRUMENT(S):

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Joern Dengjel

LAB HEAD: Joern Dengjel

PROVIDER: PXD055776 | Pride | 2025-04-10

REPOSITORIES: Pride

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			Action	DRS
	20180615_ZH_Martina_KA_all_pep_negative.raw	Raw
	20180615_ZH_Martina_KA_all_pep_positive2.raw	Raw
	20180618_evidence.txt	Txt
	20180618_msms.txt	Txt
	20180618_parameters.txt	Txt

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