Identification of di-Boron cleavage events at Dha residues on recombinant protein in e.coli lysate

ABSTRACT: Neurokinin-A (NKA) is only active when its C-terminus is amidated. B2(OH2) is thought to interact with Dha and cleave the peptide, leaving the amide on the C-terminus of the left-hand peptide. PeptideID MS/MS experiment was performed in e.coli lysate with overevpressed recombinant NKA-eH3 to prove diBoron activity in biologically relevant context. Overall analysis of the Dha generation and consumption on e.coli lysate was also performed.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Yana Demyanenko  

LAB HEAD: Ben Davis

PROVIDER: PXD030233 | Pride | 2022-05-19

REPOSITORIES: Pride