Identification of di-Boron cleavage events at Dha residues on recombinant protein in e.coli lysate
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Exploris 480
ORGANISM(S): Escherichia Coli
SUBMITTER: 
Yana Demyanenko
LAB HEAD: Ben Davis
PROVIDER: PXD030233 | Pride | 2022-05-19
REPOSITORIES: Pride
Publications
Reductive site-selective atypical <i>C</i>,<i>Z</i>-type/N2-C2 cleavage allows C-terminal protein amidation.
Mollner Tim A TA Giltrap Andrew M AM Zeng Yibo Y Demyanenko Yana Y Buchanan Charles C Oehlrich Daniel D Baldwin Andrew J AJ Anthony Daniel C DC Mohammed Shabaz S Davis Benjamin G BG
Science advances 20220408 14
Biomolecule environments can enhance chemistries with the potential to mediate and modulate self-modification (e.g., self-cleavage). While these enhanced modes are found in certain biomolecules (e.g., RNA ribozymes), it is more rare in proteins. Targeted proteolytic cleavage is vital to physiology, biotechnology, and even emerging therapy. Yet, purely chemically induced methods for the site-selective cleavage of proteins remain scarce. Here, as a proof of principle, we designed and tested a syst ...[more]
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