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Genome-wide mRNA profiling of induced Medulloblastoma (iMB) from human iPS cells

ABSTRACT: Genome-wide mRNA profiling of induced Medulloblastoma (iMB) from human iPS cells

PROVIDER: PRJNA645224 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12189662_1.fastq.gz	Fastqsanger.gz
	SRR12189662_2.fastq.gz	Fastqsanger.gz
	SRR12189663_1.fastq.gz	Fastqsanger.gz
	SRR12189663_2.fastq.gz	Fastqsanger.gz
	SRR12189664_1.fastq.gz	Fastqsanger.gz

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Genome-wide mRNA profiling of induced Medulloblastoma (iMB) from human iPS cells

Project description:We used human induced pluripotent stem cells (iPSCs) to establish tumors recapitulating the histological and transcriptional hallmarks of MYC-driven medulloblastoma (MB). We generated neuronal precursors from human iPSCs. These neuronal precursors were transformed by the MYC oncogene combined with p53 loss-of-function to generate MB xenografts for RNA-seq analysis.

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Project description:We report the application of MNase-seq (Kent, Adams et al. 2011) to construct genome-wide nucleosome maps from human cells. To date, genome-wide changes in chromatin structure that occur during development in human cells have not been investigated widely. We have constructed and compared genome-wide chromatin maps from undifferentiated human iPS cells and and iPS cells differentiated to neural progenitor cells (NPC).

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