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Response of aspen cell cultures fed piperonylic acid to methyl jasmonate.

ABSTRACT: Response of aspen cell cultures fed piperonylic acid to methyl jasmonate.

PROVIDER: PRJNA645444 | ENA |

REPOSITORIES: ENA

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Response of aspen cell cultures fed piperonylic acid to methyl jasmonate.

Project description:To better understand the role of phenylpropanoid pathway perturbation in plant metabolism, Populus tremuloides cell cultures previously established from leaf mesophyll tissue were fed with piperonylic acid (PIP) alone, or in combination with methyl jasmonate (MJ_PIP) approximately 5 days after transfer to fresh medium. Methyl jasmonate is an elicitor that activates a suite of defense responses including phenylpropanoid metabolism, while PIP acts as an inhibitor of cinnamate-4-hydroxylase (C4H). Sample tissues were contemporary with those used to generate Series GSE16773. Samples were harvested 48 hrs after initiation of the experiment. Total RNA was extracted and gene expression measured using Affymetrix poplar genome microarrays.

2022-05-06 | GSE154225 | GEO

Response of aspen cell cultures to combinatorial feeding of methyl jasmonate and AOPP.

Project description:To better understand the role of phenylpropanoid pathway perturbation in plant metabolism, Populus tremuloides cell cultures previously established from leaf mesophyll tissue were fed with methyl jasmonate, alpha-aminooxy-beta-phenylpropionic acid (AOPP), or both approximately 5 days after transfer to fresh medium. Methyl jasmonate is an elicitor that activates a suite of defense responses including phenylpropanoid metabolism. In contrast, AOPP acts as an inhibitor of phenylalanine ammonia lyase (PAL). Samples were harvested 48 hrs after initiation of the experiment. Total RNA was extracted and gene expression measured using Affymetrix poplar genome microarrays.

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Response of aspen cell cultures to combinatorial feeding of methyl jasmonate and AOPP.

Project description:Response of aspen cell cultures to combinatorial feeding of methyl jasmonate and AOPP.

| PRJNA645442 | ENA

Gene expression response of Populus tremuloides cell suspension cultures to methyl jasmonate feeding

Project description:Gene expression response of Populus cell cultures subjected to methyl jasmonate feeding was analyzed using the Affymetrix poplar genome microarrays. Keywords: Stress response

2009-12-01 | GSE16773 | GEO

Grapevine cell cultures transcriptomic response to methyl jasmonate and cyclodextrin elicitors

Project description:The specificity and synergisms of methyl jasmonate and cyclodextrin elicitor effects in grapevine were analysed at the transcrptomic level. To this end, 12-14 day old cell cultures established from Vitis vinifera L. cv Monastrell calli were elicited with 50 mM cyclodextrin (CD), 100 µM methyl jasmonate (MJ) or the combination of both elicitros (MJ+CD). The experiment was carried out in triplicate and untreated cell cultures were followed in parallel as a control. Cells of each culture were isolated just prior and 24h after the treatment to obtain RNA. GrapeGen GeneChip microarray hybridization was performed from each sample. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Pablo Carbonell-Bejerano. The equivalent experiment is VV44 at PLEXdb.]

2015-11-15 | GSE42312 | GEO

Transcription profiling of Populus tremuloides cell suspension cultures to methyl jasmonate feeding

Project description:Gene expression response of Populus cell cultures subjected to methyl jasmonate feeding was analyzed using the Affymetrix poplar genome microarrays. Experiment Overall Design: Populus tremuloides suspension cells wereÂ treated with methyl jasmonate (MJ) in DMSO orÂ with DMSO alone (C).Â Cells were harvested after 48 hours.Â Two biological replicates were obtained for each condition.

2009-12-14 | E-GEOD-16773 | biostudies-arrayexpress

Effect of Methyl jasmonate of the Poplar root transcriptome

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Multiomics analysis of Morus alba cell cultures elicited with methyl-beta-cyclodextrin and methyl jasmonate to investigate stilbene metabolism

Project description:In this study, M. alba cell suspension culture established after young stems were treated with elicitors methyl-beta-cyclodextrin combined with methyl jasmonate at 50mM and 0.1mM concentration, respectively. Elicited and control cell cultures were sampled at 0, 6, 12, 24, 48 and 72 hours and used for stilbene analysis in both the extracellular and intracellular medium, and for RNA and protein extraction. RNAseq and label-free proteomic analysis was then performed. All time point series were used for transcriptomic and 24, 48 and 72 hours time points the proteomic analysis. Transcriptomic data were used to reannotate the genome draft published by (Jiao et al. (2020), Molecular Plant, 13(7), 1001-1012) and to update the M. alba transcript and peptide database. MS-based protein identification greatly benefit from the reannotated database. A multiomics analysis was performed to search for candidates of stilbene biosynthesis in M. alba.

2026-09-07 | PXD048037 | Pride

Effect of Methyl jasmonate of the Poplar root transcriptome

Project description:Illumina HiSeq technology was used to generate mRNA profiles from Populus tremula x alba INRA 717-1B4 roots treated with Methyl jasmonate. Samples were harvested after two weeks either from untreated control roots or from Methyl jasmonate treated roots. Paired-end (2X100bp) reads were generated and aligned to the Populus trichocarpa (http://www.phytozome.net/poplar.php) using CLC Genomics Workbench 6. mRNA profiles from Populus tremula x alba INRA 717-1B4 roots treated with Methyl jasmonate as well as from control roots were generated by paired-end (2X100bp) Illumina HiSeq sequencing. Four samples were sequenced per lane, two biological replicates per treatment.

2014-04-17 | E-GEOD-56864 | biostudies-arrayexpress

Comparative transcriptome analysis of rice roots treated with the resistance inducers piperonylic acid, acibenzolar-S-methyl and beta-aminobutyric acid

Project description:We used mRNA sequencing to study the effects of foliar treatment with piperonylic acid, acibenzolar-S-methyl or beta-aminobutyric acid on the root transcriptome of rice plants (cv. Kitaake) in order to find conserved systemic responses to distinct resistance-inducing stimuli

2022-12-06 | GSE194400 | GEO

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