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Premature activation of immune transcription programs in autoimmune-predisposed mouse embryonic stem cells and blastocysts

ABSTRACT: Premature activation of immune transcription programs in autoimmune-predisposed mouse embryonic stem cells and blastocysts

PROVIDER: PRJNA646374 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12224941.fastq.gz	Fastqsanger.gz
	SRR12224942.fastq.gz	Fastqsanger.gz
	SRR12224943.fastq.gz	Fastqsanger.gz
	SRR12224944.fastq.gz	Fastqsanger.gz

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Premature activation of immune transcription programs in autoimmune-predisposed mouse embryonic stem cells and blastocysts

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Premature activation of immune transcription programs in autoimmune-predisposed mouse embryonic stem cells and blastocysts

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Premature activation of immune transcription programs in autoimmune-predisposed mouse embryonic stem cells and blastocysts

Project description:To study the underlying mechanism of autoimmunity predisposition in autoimmune-prone mouse strains, we characterize embryonic stem cells derived from various mouse strains.

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Premature activation of immune transcription programs in autoimmune-predisposed mouse embryonic stem cells and blastocysts

Project description:To study the underlying mechanism of autoimmunity predisposition in autoimmune-proned mouse strains, we characterize embryonic stem cells derived from various mouse strains.

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Premature activation of immune transcription programs in autoimmune-predisposed mouse embryonic stem cells and blastocysts

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Premature Activation of Immune Transcription Programs in Autoimmune-Predisposed Mouse Embryonic Stem Cells and Blastocysts.

Project description:Autoimmune diabetes is a complex multifactorial disease with genetic and environmental factors playing pivotal roles. While many genes associated with the risk of diabetes have been identified to date, the mechanisms by which external triggers contribute to the genetic predisposition remain unclear. Here, we derived embryonic stem (ES) cell lines from diabetes-prone non-obese diabetic (NOD) and healthy C57BL/6 (B6) mice. While overall pluripotency markers were indistinguishable between newly derived NOD and B6 ES cells, we discovered several differentially expressed genes that normally are not expressed in ES cells. Several genes that reside in previously identified insulin-dependent diabetics (Idd) genomic regions were up-regulated in NOD ES cells. Gene set enrichment analysis showed that different groups of genes associated with immune functions are differentially expressed in NOD. Transcriptomic analysis of NOD blastocysts validated several differentially overexpressed Idd genes compared to B6. Genome-wide mapping of active histone modifications using ChIP-Seq supports active expression as the promoters and enhancers of activated genes are also marked by active histone modifications. We have also found that NOD ES cells secrete more inflammatory cytokines. Our data suggest that the known genetic predisposition of NOD to autoimmune diabetes leads to epigenetic instability of several Idd regions.

| S-EPMC7460978 | biostudies-literature

Premature activation of immune transcription programs in autoimmune-predisposed mouse embryonic stem cells and blastocysts

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-08-11 | GSE155046 | GEO

BSO-treated blastocysts vs CTL-treated blastocysts

Project description:In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act intra-cellularly by inhibiting GSH synthesis (0.4 mM buthionine sulfoximine [BSO]) was added from days 3 to 7, and transcriptomic analysis was then performed in resulting blastocysts. Precisely, after in vitro maturation and fertilization, bovine zygotes were culture in vitro in normal condition, then at day 3, embryos were allocated into culture in control or supplemented with BSO (0.4 mM) until day 7. At this time, blastocysts were harvested and analyzed. Our hypothesis was that BSO treatment will affect blastocyst survival and gene expression associated with low embryo quality

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AAPH-treated blastocysts vs CTL-treated blastocysts

Project description:In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act extra-cellularly by promoting ROS production (0.01 mM 2,2'-azobis (2-amidinopropane) dihydrochloride [AAPH]) was added from days 3 to 7, and transcriptomic analysis was then performed in resulting blastocysts. Precisely, after in vitro maturation and fertilization, bovine zygotes were culture in vitro in normal condition, then at day 3, embryos were allocated into culture in control or supplemented with AAPH (0.01 mM) until day 7. At this time, blastocysts were harvested and analyzed. Our hypothesis was that AAPH treatment will affect blastocyst survival and gene expression associated with low embryo quality

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