Project description:Temperature stress is a survival challenge that biological cells may encounter at any time. To sustain normal metabolic activities, organisms have gradually evolved strategies to respond promptly to temperature changes. The two-component system, typically involved in regulating the transcription of relevant genes in response to environmental stimuli, consists of a histidine kinase and a response regulator. This system mediates environmental signal transduction in microbial cells. This study identified DhqSR as a novel temperature-sensing two-component system in Thermus thermophilus HB27. It regulates the expression of type II 3-dehydroquinate dehydratase (DHQase) in the aromatic amino acid biosynthesis pathway, indirectly controlling aromatic amino acid synthesis and thereby altering cellular thermal adaptation capabilities.
Project description:Immune precipitation was performed using TtAgo antibody on Thermus thermophilus HB27 wild-type (WT) and ago gene deletion mutant （Δago）grown to logarithmic phase (OD600 = 0.5). The precipitated proteins were subjected to mass spectrometry analysis
