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Gene expression signatures of CD11b+ cells in mice after myocardial infarction

ABSTRACT: Gene expression signatures of CD11b+ cells in mice after myocardial infarction

PROVIDER: PRJNA647355 | ENA |

REPOSITORIES: ENA

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Gene expression signatures of CD11b+ cells in mice after myocardial infarction

Project description:To further search for potential gene targets which are involved in the pathological process of myocardial infarction, we have employed whole genome microarray expression profiling as a discovery platform to identify the expression of STAT6, β1-adrenergic receptors and inflammatory cytokines. The CD11b+ cells were isolated from spleen and bone marrow of mice by magnetic beads, and the experimental mice were divided into four time points (control, MI 1week, MI 2 weeks, and MI 4 weeks). We found the β1-AR related sympathetic signal, the STAT6 signal and inflammatory cytokines (IL-1α, IL-18 and TGF-β) are involved in the activation of CD11b+ immune cells and cardiac fibrogenesis after myocardial infarction.

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myocardial infarction in Reg3beta knock-out mice

Project description:Affymetrix microarray analysis of molecular changes after myocardial infarction. Samples of heart tissue were analyzed after myocardial infarction from WT and reg3beta knock-out mice. Samples from scar tissue and samples adjacent to the scar were analyzed. In the experiment we primarily compared infarction zone of wild-type to infarction zone of knock-out animals, and remote zone of wild-type to remote zone of knock-outs.

2015-06-29 | E-MTAB-2561 | biostudies-arrayexpress

Time series single cell transcriptomics of cardiac inflammation after myocardial infarction in mice

Project description:We performed time-series single-cell RNA-seq analysis of blood CD11b+ cells and of the inflammatory infiltrate in the heart of C57BL/6J male mice in a model of permanent myocardial infarction.

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Project description:Background and Aims: It is known that inflammatory processes are activated in heart failure, but the regulation of cytokines and their role in the pathogenesis of the disease are not well understood. To address this issue, we have performed microarray analysis of non-infarcted left ventricular tissue from mice at various time-points after myocardial infarction. Methods: Molecular alterations in myocardial tissue were measured 3, 5, 7 and 14 days after induced infarction by using cDNA microarrays. Sham operated mice served as controls. Altered gene transcriptions were verified by real-time polymerase chain reaction. Attention focused on genes encoding cytokines which had not previously been assigned a role in heart failure development. Results: The highest number of regulated genes was found at day 5 post myocardial infarction, and 22 genes encoding cytokines were identified as being regulated. Several of the identified genes encoding cytokines have not previously been associated with HF, and among those fractalkine showed strongest up-regulation. Keywords: Disease state analysis, time course

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Transcriptome analysis of CD11b+ myeloid cells isolated from the infarction area of Reg3beta knock-out Reg3beta-/- vs. WT mice

Project description:Adult wild-type (C57/Bl6J) and mice with constitutive knock-out of regenerating-islet derived protein 3 beta (Reg3beta-/-) at the age of 8-12 weeks were subjected to experimental myocardial infarction (LAD ligation). After 4 days, infarcted tissue was dissected, minced with scissors and enzymatically digested with liberase blendzyme (Roche, 0.15 mg/ml) to obtain a single-cell suspension. Isolated cells were washed and resuspended in PBS buffer supplemented with 0.5 % BSA and 2 mM EDTA and passed through 30 um pre-separation filters. The myeloid cells were isolated using the mouse/human CD11b Microbead Kit (Miltenyi Biotec, 130-049-061) and RNA was isolated using the TRIzol method (Invitrogen).

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Experimentally induced myocardial infarction in mice - RNA Sequencing of kidneys

Project description:To induce myocardial infarction, ligation of left anterior descending (LAD) coronary artery was performed for 5d and 28d. Kidneys were collected from MI mice and sham-operated mice (5d).

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Project description:Dynamic molecular signatures of acute myocardial infarction based on transcriptomics

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The effect of insulin-like growth factor 1 treatment on cardiac myeloid cells 3 days post-myocardial infarction

Project description:To test the mechanism by which IGF1 is cardioprotective, we performed single cell RNA sequencing on myeloid cells isolated from the heart 3 days after myocardial infarction of mice with and without IGF1 treatment. Myocardial infarction was induced in C57Bl6/J mice by 45 min occlusion of the left anterior descending artery followed by 3 days of reperfusion. Animals of the IGF1 group (n=3) received 40 ng/g mature recombinant IGF1 subcutaneously as bolus at the beginning of reperfusion. In addition, IGF1 (1 µg/g/d) was administered continuously during reperfusion using micro-osmotic pumps (Alzet, 1003D) that were implanted subcutaneously. Control mice received vehicle (0.1% BSA). After 3 days hearts were digested and CD45+CD11b+ cells were isolated using FACS cell sorting. Each sample contained cells containing 1 control and 1 IGF1 treated mouse, labeled with TotalSeq hashtags. 16000 cells were used as input for the single-cell droplet libraries generation for each sample.

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2022-12-31 | GSE198301 | GEO

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