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Clca1 expression in the aged kidney in mice

ABSTRACT: Clca1 expression in the aged kidney in mice

PROVIDER: PRJNA649595 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12347711_1.fastq.gz	Fastqsanger.gz
	SRR12347711_2.fastq.gz	Fastqsanger.gz
	SRR12347712_1.fastq.gz	Fastqsanger.gz
	SRR12347712_2.fastq.gz	Fastqsanger.gz
	SRR12347713_1.fastq.gz	Fastqsanger.gz

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Clca1 expression in the aged kidney in mice

Project description:In this study, a discovery approach was designed to identify novel mRNA transcripts that may be involved in kidney aging. RNA-Seq revealed increase in the expression of Clca1 mRNA in the aged kidney. CLCA1 facilitates Cl- current operated by TMEM16A, a Ca++-dependent Cl- channel. Cl- movement accompanied by cations such as Na+ and K+ or in exchange for other anions occurs among fluid compartments in many segments of the nephron. Cl- shift among these compartments is governed by electrochemical potential and transporters and exchangers.

2021-06-01 | GSE155407 | GEO

Global analysis of kidney gene expression by RNA-Seq of aged CTRP1-deficient mice

Project description:Aging affects multi-organ systems. What local and systemic factors influence renal structure and function in aging are not well understood. We and others have shown that the secretory protein, C1q/TNF-related protein 1 (CTRP1), regulates systemic metabolism and cardiovascular function. Whether CTRP1 has a role in kidney function is unknown. We provide evidence here that CTRP1 modulates renal physiology in an age- and sex-dependent manner. In addition to adipose tissue, CTRP1 is also expressed in kidney glomeruli, with podocyte being a major cell source. In mice lacking CTRP1, we observed significant increase in kidney weight and glomeruli hypertrophy in aged (~1 year) male, but not female or younger mice. Although glomerular filtration rate, plasma renin and aldosterone levels, and renal response to water restriction are not different between genotypes, aged CTRP1-deficient male mice have elevated blood pressure. Echocardiogram and pulse wave velocity measurements indicate normal heart function and vascular stiffness in CTRP1 knockout animals. Increased blood pressure is not due to greater salt retention. Paradoxically, CTRP1-deficient mice have elevated urinary sodium and potassium excretion, resulting, in part, from reduced expression of genes involved in renal sodium and potassium reabsorption. Despite renal hypertrophy, gene expression related to inflammation, fibrosis, and oxidative stress are significantly lower in CTRP1-deficient mice. RNA sequencing and pathway analyses reveal additional alterations and enrichments of genes in metabolic processes in the kidney of CTRP1-null animals. These results highlight novel contributions of CTRP1 to aging-associated changes in renal structure and function

2020-12-22 | GSE127332 | GEO

Global analysis of kidney gene expression by RNA-Seq of aged CTRP1-deficient mice

Project description:Global analysis of kidney gene expression by RNA-Seq of aged CTRP1-deficient mice

| PRJNA524700 | ENA

Transcription profiling of liver, lung, spleen and kidney from young (13 week) and aged (130 week) wild type mice

Project description:We compared the complete mouse liver, spleen, lung and kidney transcriptome of adult 13- and 130-wk old wt C57Bl/6J mice (n=3) to develop gene sets for young adult and aged wt mice, respectively

2008-06-01 | E-MEXP-1504 | biostudies-arrayexpress

Global transcriptomic changes in aged mouse kidney podocytes

Project description:Like many cell types, the mechanisms and pathways underlying healthy aged-related changes to podocytes are not fully understood. Candidate pathways include oxidative stress, epigenetic changes, senescence, sirtuins, reduced autophagy and increased apoptosis, although detailed mechanisms underlying each pathway is not well defined. While detailed gene analysis has been undertaken on whole portions of the aging kidney, transcriptomic changes specific to podocytes in the aged kidney are not known. To address this, we used an inducible podocyte specific reporter mouse in which a cohort of podocytes are permanently labeled over the life time of the animal. RNA-seq was then used to measure transcriptional changes in genes in labeled podocytes in mice with advanced age, compared to podocytes from a cohort of young reporter mice.

2020-08-20 | GSE136138 | GEO

Protein expression in the prefrontal cortex of aged mice with progranulin deficiency or neuronal progranulin overexpression

Project description:Description: Progranulin deficiency is associated with neurodegeneration in humans and in mice. We performed a deep proteomic screen of the pre-frontal cortex in aged (13-15 months) female progranulin-deficient mice (GRN-/-) and mice with a neuron-specific inducible overexpression of progranulin (SLICK-GRN-OE with tamoxifen) versus the respective control mice (Grn+/+ and SLICK-Grn without Tamoxifen). The data set shows progranulin-dependent alterations of protein expression in the cortex of mice.

2016-10-13 | PXD004087 | Pride

Global analysis of kidney gene expression by RNA-Seq of aged CTRP1-deficient mice on high-fat diet

Project description:Global analysis of kidney gene expression by RNA-Seq of aged CTRP1-deficient mice on high-fat diet

| PRJNA634907 | ENA

Aging and senescence-associated signatures of aged kidney glomerular cells [scRNA-seq]

Project description:The kidney governs many physiological factors of the body, and the glomerulus is its core filtration unit. Glomeruli comprise of less than 1.5% of the kidney’s volume and thus are underrepresented in many analyses of the kidney, and require particular isolation methods. Aging is a major risk factor for kidney disease, yet despite the importance of proper glomerular function, we have limited knowledge of the changes that glomerular cells undergo during aging. Here we interrogate the aged mouse kidney, glomerulus and glomerular cells using various approaches, including single cell RNA sequencing (scRNA-Seq), to identify age and senescence associated changes at the tissue and cellular level. We show that the aged kidney and glomerulus undergo structural changes, including increased fibrosis, glomerulonephritis and expression of injury and senescence-associated markers. Lastly, we utilized our method for high-throughput analysis of mouse glomeruli to characterize cell-specific and age-related changes in glomerular cells. This analysis revealed a senescence-associated phenotype in aged mesangial cells, and emphasized the potential importance of mesangial cells and macrophages in this niche. Our study provides insights into the molecular and cellular changes of the aging glomerulus that suggest therapeutic targets for age-related renal dysfunction.

2024-09-01 | GSE240374 | GEO

Global transcriptomic changes in aged mouse kidney podocytes

Project description:Global transcriptomic changes in aged mouse kidney podocytes

| PRJNA561363 | ENA

Global analysis of kidney gene expression by RNA-Seq of aged CTRP1-deficient mice on high-fat diet

Project description:Aging and obesity profoundly influence organ system health by altering circulating levels of hormones, growth factors, and cytokines. C1q/TNF-related protein 1 (CTRP1) is an endocrine factor with important metabolic, cardiovascular, and renal functions. We sought to determine the impact of CTRP1 deficiency on heart and kidney function in the context of aging and obesity using aged and obese wild-type (WT) and Ctrp1 knockout (KO) mice fed a high-fat diet for eight months or longer. Surprisingly, no differences were observed in blood pressure, heart function, or vascular stiffness between genotypes. Loss of CTRP1, however, resulted in renal enlargement (relative to body weight), reduced urinary creatinine, elevated urinary total protein content and pH, and changes in renal gene expression affecting sugar, fat, and amino acid metabolism pathways. Assessment of glomerular integrity, the extent of podocyte foot process effacement, as well as renal response to water restriction and salt loading did not reveal significant differences between WT and Ctrp1-KO mice. Interestingly, blood platelet, white blood cell, neutrophil, lymphocyte, and eosinophil counts were significantly elevated, whereas mean corpuscular volume and hemoglobin were reduced in Ctrp1-KO mice. Cytokine profiling revealed increased circulating levels of CCL17 and TIMP-1 in KO mice. Compared to a previous study, our current data suggest that chronic high-fat feeding affects renal phenotypes differently than similarly aged mice fed a control low-fat diet, highlighting a diet-dependent contribution of CTRP1 deficiency to age-related changes in renal structure and function.

2020-10-13 | GSE151154 | GEO

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