Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis in young and senescent endothelial cells

ABSTRACT: RNA-seq analysis in young and senescent endothelial cells

PROVIDER: PRJNA655211 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12385885.fastq.gz	Fastqsanger.gz
	SRR12385886.fastq.gz	Fastqsanger.gz
	SRR12385887.fastq.gz	Fastqsanger.gz
	SRR12385888.fastq.gz	Fastqsanger.gz
	SRR12385889.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

MicroRNA profiling of human umbical vein endothelial cells (HUVEC) using young (replicating) cells and senescent cells

Project description:From two donors of human umbilical vein endothelial cells, in vitro cell lines were established. Both cell lines were grown in vitro until irreversible growth arrest was observed (replicative senescence). Total RNA from young (replicating) cells as well as senescent cells was harvested and used for hybridization of microRNA chips (MRC) from TU Graz based on Sanger miRBase 9.2

2010-04-19 | E-MEXP-2425 | biostudies-arrayexpress

MicroRNA signature of young and replicative senescent human umbilical vein endothelial cells (HUVECs)

Project description:MicroRNAs regulate various cellular processes. While several genes associated with replicative senescence have been described in endothelial cells, miRNAs that regulate these genes remain largely unknown. The present study was designed to identify miRNAs associated with replicative senescence and their target genes in HUVECs. We have employed Agilent Human MicroRNAs microarray platform to evaluate the expressions of 866 human miRNAs and 89 human viral miRNAs, based on Sanger miRNA database release 12.0 miRNA expression profiles were established for young and replicative senescent HUVECs

2013-04-05 | E-GEOD-37092 | biostudies-arrayexpress

Gene signature of young and replicative senescent human umbilical vein endothelial cells (HUVECs)

Project description:MicroRNAs regulate various cellular processes. While several genes associated with replicative senescence have been described in endothelial cells, miRNAs that regulate these genes remain largely unknown. The present study was designed to identify miRNAs associated with replicative senescence and their target genes in HUVECs. Gene profiling was established using the same RNA input as that used for miRNA profiing. We have employed Agilent Whole Human Genome microarray platform to evaluate the expressions of 19,596 human genes . Gene expression profiles were established for young and replicative senescent HUVECs

2013-04-05 | E-GEOD-37091 | biostudies-arrayexpress

RNA-seq of young and senescent human umbilical vein endothelial cells (HUVECs) under normal or high glucose conditions

Project description:Human endothelial cellular models are useful to disentangle the pathophysiological role of dysfunctional endothelium in the development of cardiovascular (CV) disease and organ damage in T2D. Here, we performed a RNAseq of human umbilical vein endothelial cells (HUVECs) undergoing replicative senescence and exposed to high glucose (25 mM) to investigate the combined effects of replicative senescence and high glucose on the transcriptional landscape of these cells. To gain insight into the molecular mechanisms driving the acquisition of a senescent phenotype following exposure to HG, we performed a RNA-seq assay on control (Ctr) and replicative senescent (Sen) HUVECs cultivated in presence/absence of HG culture medium (total number of samples = 12; number of samples in each cell type-medium condition group = 3) using the NovaSeq 6000 Illumina system. Differential expression analysis was performed in R environment (version 4.2.2) using the limma and edgeR Bioconductor R packages.

2024-04-08 | E-MTAB-13959 | biostudies-arrayexpress

RNA-seq analysis in young and senescent endothelial cells

Project description:To investigate how the pattern of gene expression is changed in endothelial cells by cellular senescence, RNA-seq analysis was carried out with human umbilical vein endothelial cells(HUVECs)

2021-10-25 | GSE155680 | GEO

Mouse embryonic fibroblasts (MEFs): Senescent cells vs Young cells

Project description:LncRNAs and mRNAs expression profile of MEFs comparing senescent cells with young cells. The microarray with coverage of 41597 mouse lncRNAs and 35291 mouse mRNAs. In the study presented here, six samples were analyzed. Biological replicates: three Senescent MEFs, three Young MEFs, independently grown and harvested.

2015-10-29 | E-GEOD-74435 | biostudies-arrayexpress

Young Human Plasma Rejuvenates Senescent Vascular Endothelial Cell through Multiple Pathways

Project description:A mouse parabiotic model supports the existence of transferable factor(s) in blood that rescue(s) age-related degenerative phenotypes, however, whether it prevents endothelial cell senescence linked to common cardio-cerebral vascular diseases, requires to be inspected. In this study, plasmas donated from 27 young (23.04 ± 4.86 years, young human plasma, YHP) and 26 elderly (55.04 ± 5.42 years, elderly human plasma, EHP) healthy volunteers were used to culture primary human umbilical endothelial cells (HUVEC). When comparing with EHP, we found that the YHP reduced the percentages of SABG-stained cells and prohibited the expressions of senescent markers, including p16, p21, and p53 in HUVEC. Functionally, the YHP decreased the expression of ICAM-1, a marker for endothelial cell activation and increased abilities in tube formation and mobility at late passages that represent an enhanced angiogenic function. Validated by both RNA sequencing and Real-time PCR in HUVECs from all three umbilical cords, nineteen up-regulated and fifteen down-regulated genes were observed in YHP-treated HUVEC compared with group treated with the EHP. Among 34 genes, the expressions of MCM10, DHCR24, WBP1, UNC5B, and CYP26B1 were reversed by the YHP and we showed that these reversed expressions delayed senescence of both HUVEC and human coronary artery endothelial cell (HCAEC) through p16- and/or p21-assocated signaling. The five genes are relevant to maintain or reinstate the ability for proliferation, anti-apoptosis, and endothelial cell related functions, such as angiogenesis and anti-atherosclerosis. Thus, our study suggests that YHP restores biological processes in senescent endothelial cell through multiple pathways.

2021-12-31 | GSE140592 | GEO

Genome-wide expression analysis of young, senescent and p38MAPK-inhibitited senescent human fibroblasts.

Project description:We utilized whole genome sequencing of mRNA (RNA-seq) to understand the extent to which the senescence-associated secretory phenotype is regulated by p38MAPK Examination of replicates of young, senescent or p38MAPK-inhibited senescent BJ human foreskin fibroblasts.

2014-04-01 | E-GEOD-56293 | biostudies-arrayexpress

Young Human Plasma Rejuvenates Senescent Vascular Endothelial Cell through Multiple Pathways

Project description:Young Human Plasma Rejuvenates Senescent Vascular Endothelial Cell through Multiple Pathways

| PRJNA590216 | ENA

Transcription profiling by array of human young and senescent HUVECs under static and laminar shear stress conditions

Project description:Laminar shear stress due to constant blood flow is known to play a critical role in maintaining vascular health. In contrast, endothelial cell senescence appears to be closely associated with the incidence of vascular disorder. In an attempt to identify functional biomarkers for age-related vascular health/disease, the present study investigated differential gene expression of young and senescent human umbilical vein endothelial cells (HUVECs) under static and laminar shear stress. We used a cDNA microarray method to compare gene expression profiles of young and senescent HUVECs under static and laminar shear stress conditions. Experiment Overall Design: Senescent cells were prepared by continuous subculture in vitro, and a cone-and-plate device was used to impose laminar shear stress onto cells. Young and senescent cells were exposed to laminar shear stress or maintained under static conditions. Total mRNA was extracted and gene expression profiles were analyzed by cDNA microarray.

2009-06-01 | E-GEOD-13712 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data